Changes in oxidative stress and lipoprotein in malnourished gerbils infected with Giardia lamblia.
Changes in oxidative stress and lipoprotein in malnourished gerbils infected with Giardia lamblia.
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DOI：
10.5530/fra.2014.1.10
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
de Oliveira, D. R.
中科院分区：
文献类型：
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作者：
Ventura, L. L. A.;Caliari, M. V.;de Oliveira, D. R.
简介： 据推测，贾第鞭毛虫病是世界上最常见的寄生虫之一，它会产生代谢变化，使营养不良或免疫功能低下的患者的营养状况恶化。在这种情况下，我们试图通过评估感染对先前营养不良的沙鼠的抗氧化防御和脂蛋白代谢的影响来帮助澄清这个问题。方法：使用 32 只沙鼠，分为四组：对照组（CT）和感染对照组（CTIn），每组接受 20% 蛋白质饮食；营养不良组（MN）和营养不良感染组（MNIn），每组接受 5% 蛋白质饮食。 CTIn和MNIn组接种1×106个G.lamblia滋养体，其余组进行模拟感染。感染后7天，处死所有组并收集肝组织以通过给药过氧化氢酶和超氧化物歧化酶来评估氧化应激，并收集血清以通过快速蛋白液相色谱评估脂蛋白代谢。在每个级分中，使用商业试剂盒测定胆固醇浓度，并通过吸光度差异测定蛋白质浓度。结果：低蛋白饮食喂养的沙鼠体重显着降低。与 CT 相比，MNIn 组中级分中的胆固醇和蛋白质显着降低，并且与对照组相比，MNIn 组中过氧化氢酶活性更高。结论：我们的结果表明，宿主对与营养不良相关的贾第鞭毛虫病的反应会导致氧化应激，从而导致营养不良动物的脂蛋白组成发生变化，表明感染可能影响代谢变化，使受感染动物的营养状况恶化。
Introduction: It is speculated that giardiasis, one of the most common parasites in the world, would produce metabolic changes that worsen the nutritional status of malnourished or immunocompromised patients. In this context, we seek to contribute to clarify this issue by evaluating the effects of infection on antioxidant defense and lipoprotein metabolism in gerbils previously malnourished. Methods: 32 gerbils were used, divided into four groups: Control (CT) and Control Infected (CTIn), which each received a 20% protein diet, Malnourished (MN) and Malnourished Infected (MNIn), which each received a 5% protein diet. CTIn and MNIn groups were inoculated with 1*106 trophozoites of G. lamblia, while the remaining groups were mock infected. Seven days post-infection, all groups were sacrificed and hepatic tissue collected for assessment of oxidative stress via dosing catalase and superoxide dismutase and serum was collected to evaluate the lipoprotein metabolism by Fast Protein Liquid Chromatography. In each fraction, cholesterol concentration was determined using commercial kits, and protein by absorbance difference. Results: Gerbils fed with a low protein diet had significantly lower body weight. Cholesterol and protein in the fractions were significantly lower in group MNIn compared to CT and Catalase activity was higher in group MNIn compared to control. Conclusions: Our results demonstrated that host response against giardiasis associated to malnutrition, led to an oxidative stress that produced changes on lipoprotein composition in the malnourished animals suggesting that the infection could influence on metabolic changes, worsening the nutritional status of infected animals.