Biochemical effects of some pesticides on lipid peroxidation and free-radical scavengers
Biochemical effects of some pesticides on lipid peroxidation and free-radical scavengers
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DOI：
10.1016/s0378-4274(99)00029-6
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发表时间：
1999-06-30
影响因子：
3.5
通讯作者：
Chakraborty, AK
中科院分区：
文献类型：
--
作者：
Banerjee, BD;Seth, V;Chakraborty, AK
对德里古鲁特格巴哈杜尔医院收治的林丹、马拉硫磷和残杀威中毒病例的血液样本进行了氧化应激研究，并对其进行了脂质过氧化、氧自由基(OFR)清除酶和谷胱甘肽(GSH)及相关酶的评估。检测淋巴细胞中乙酰胆碱酯酶(AChE)、γ-谷氨酰转肽酶(GGT)和谷胱甘肽(GSH)水平。农药中毒时，硫代巴比妥酸反应物质含量增加，超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽-S转移酶、谷胱甘肽转移酶活性升高，谷胱甘肽含量降低。显然，林丹(在所考察的浓度下)在脂质过氧化、GSH相关参数和OFR清除酶方面比马拉硫磷和残杀威产生更强的改变。与对照组相比，马拉硫磷中毒患者淋巴细胞AChE活性和GSH水平降低，GGT活性升高。本研究结果提示，在有机氯、有机磷和氨基甲酸酯中毒过程中，机体在对抗氧化应激的同时，产生了不同的氧自由基清除酶。脂质过氧化增加，加上血液中GSH和OFR清除酶水平的改变，从氧化应激的角度进行了讨论。(C)1999爱思唯尔爱尔兰科学有限公司。保留所有权利。
Oxidative stress was studied in blood samples obtained from lindane, malathion and propoxur poisoning cases admitted to the Guru Teg Bahadur Hospital, Delhi and evaluated for lipid peroxidation, oxygen free radical (OFR) scavenging enzymes, and glutathione (GSH) and related enzymes. Acetylcholine esterase (AChE), gamma glutamyl transpeptidase (GGT) and GSH level were also assayed in lymphocytes. The level of thiobarbituric acid reacting substances and activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and GGT were increased and GSH level was decreased in pesticide poisoning. Apparently lindane (at the concentration examined) was more potent than malathion and propoxur in producing alteration in lipid peroxidation, GSH related parameters and OFR scavenging enzymes. However, AChE activity and GSH level in lymphocytes of malathion poisoning cases were reduced and GGT activity was enhanced in comparison to control subjects. The present results suggest that OFR scavenging enzymes were induced while combating oxidative stress in a differential manner in organochlorine, organophosphate and carbamate poisoning. Increased lipid peroxidation, coupled with altered levels of GSH and OFR scavenging enzymes in the blood are discussed in the light of oxidative stress. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.