Bioleaching and immobilizing of copper and zinc using endophytes coupled with biochar-hydroxyapatite: Bipolar remediation for heavy metals contaminated mining soils.
Bioleaching and immobilizing of copper and zinc using endophytes coupled with biochar-hydroxyapatite: Bipolar remediation for heavy metals contaminated mining soils.
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DOI：
10.1016/j.chemosphere.2022.137730
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发表时间：
2023-01
期刊：
影响因子：
8.8
通讯作者：
Weiran Feng;Xiao Xiao-Xiao;Junjie Li;Qicheng Xiao;Li Ma;Qifeng Gao;Yuke Wan;Yutian Huang;Ting Liu;Xubiao Luo;S. Luo;G. Zeng;Kai Yu
中科院分区：
文献类型：
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作者：
Weiran Feng;Xiao Xiao-Xiao;Junjie Li;Qicheng Xiao;Li Ma;Qifeng Gao;Yuke Wan;Yutian Huang;Ting Liu;Xubiao Luo;S. Luo;G. Zeng;Kai Yu
铜和锌是土壤中的有毒重金属，需要制定可行的策略来修复矿区周围受污染的土壤。研究了固定化和生物浸出修复重金属高污染土壤的工艺条件和机理。采用生物浸出-固定化双极法进行土壤修复。结果表明，LSE03细菌对Cu2+和Zn2+具有较好的浸出效果和固定化效果。细菌代谢产物中，顺式、顺式粘膜酸和异戊酸在生物浸出过程中起主要作用。细菌胞外聚合物质中富含多种有机酸，这些有机酸经过吸附过程后含量显著降低，说明这些物质都参与了重金属的结合。利用FTIR、TEM-mapping和XPS技术对内生菌和固定化剂进行了表征，揭示了细菌和复合材料吸附Cu-Zn的能力以及- oh、- cooh、- po43 -和- nh的主要官能团。根据重金属形态分析、竞争吸附实验和生物浸出解吸实验，拟对污染土壤进行固定化-生物浸出双极性修复。双极修复处理后，有效Cu和Zn的去除率分别为97.923%和96.387%。在所有情况下，土壤肥力都显著提高。本研究为高浓度重金属污染土壤提供了一种绿色、实用、环保的处理方法。
Copper and zinc are toxic heavy metals in soils that require development of feasible strategies for remediation of contaminated soils around the mine areas. In this study, the processing conditions and mechanisms of immobilization and bioleaching for remediation of highly contaminated soils with heavy metals are investigated. Soil remediation is carried out using a bioleaching-immobilization bipolar method. The results show that LSE03 bacteria provide efficient leaching result and immobilization on Cu2+and Zn2+. Among the bacterial metabolites,cis,cis-muconic acid and isovaleric acid play major roles in the bioleaching process. The bacterial extracellular polymeric substances are rich in a variety of organic acids that show a significant decrease in content after the adsorption process, indicating that all of these substances are involved in the binding of heavy metals. Characterization of the endophytes and immobilizing agents with FTIR, TEM-mapping, and XPS techniques reveal the ability of both bacteria and composites to adsorb Cu–Zn as well as the main functional groups of –OH, –COOH, –PO43–, and –NH. According to the heavy metals species analyses, competitive adsorption experiments, and bioleaching desorption experiments, it is planned to carry out the bipolar remediation of contaminated soil through immobilization followed by bioleaching process. After bipolar remediation processing, 97.923% and 96.387% of available Cu and Zn are respectively removed. Soils fertility significantly increases in all cases. Our study provides a green, practical, and environmentally friendly treatment method for soils contaminated with high concentrations of heavy metals.