High-fat diet decreases energy expenditure and expression of genes controlling lipid metabolism, mitochondrial function and skeletal system development in the adipose tissue, along with increased expression of extracellular matrix remodelling- and inflammation-related genes
High-fat diet decreases energy expenditure and expression of genes controlling lipid metabolism, mitochondrial function and skeletal system development in the adipose tissue, along with increased expression of extracellular matrix remodelling- and inflammation-related genes
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DOI：
10.1017/s0007114515000100
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发表时间：
2015-03-28
影响因子：
3.6
通讯作者：
Jung, Un Ju
中科院分区：
文献类型：
--
作者：
Choi, Myung-Sook;Kim, Young-Je;Jung, Un Ju
本研究的目的是确定差异表达的基因在内脏脂肪组织中的一个良好的表征高脂饮食（HFD）诱导的肥胖症的小鼠模型。雄性C57 BL/6 J小鼠（n 20）喂食HFD（189%的能量来自脂肪）或低脂饮食（LFD，12%的能量来自脂肪）16周。HFD喂养的小鼠表现出肥胖、胰岛素抵抗、血脂异常和脂肪胶原积累，沿着较高水平的血浆瘦素、β-内酰胺酶和纤溶酶原激活物抑制剂1型，尽管血浆细胞因子水平没有显著差异。由于HFD组的食物摄入量较低，两个饮食组的能量摄入量相似;然而，HFD组的能量消耗也低于LFD组。基因芯片分析显示，与脂肪分解、脂肪酸代谢、线粒体能量转导、氧化还原、胰岛素敏感性和骨骼系统发育相关的基因在HFD喂养的小鼠中下调，与细胞外基质（ECM）成分、ECM重塑和炎症相关的基因上调。前十位上调或下调的基因包括Acsm 3、mt-Nd 6、Fam 13 a、Cyp 2 e1、Rgs 1和Gpnmb，其在肥胖相关脂肪组织恶化中的作用知之甚少。总之，这里确定的基因为预防和治疗饮食诱导的肥胖提供了新的治疗机会。
The aim of the present study was to identify the genes differentially expressed in the visceral adipose tissue in a well-characterised mouse model of high-fat diet (HFD)-induced obesity. Male C57BL/6J mice (n 20) were fed either HFD (189% of energy from fat) or low-fat diet (LFD, 12% of energy from fat) for 16 weeks. HFD-fed mice exhibited obesity, insulin resistance, dyslipidaemia and adipose collagen accumulation, along with higher levels of plasma leptin, resistin and plasminogen activator inhibitor type 1, although there were no significant differences in plasma cytokine levels. Energy intake was similar in the two diet groups owing to lower food intake in the HFD group; however, energy expenditure was also lower in the HFD group than in the LFD group. Microarray analysis revealed that genes related to lipolysis, fatty acid metabolism, mitochondrial energy transduction, oxidation-reduction, insulin sensitivity and skeletal system development were down-regulated in HFD-fed mice, and genes associated with extracellular matrix (ECM) components, ECM remodelling and inflammation were up-regulated. The top ten up-or down-regulated genes include Acsm3, mt-Nd6, Fam13a, Cyp2e1, Rgs1 and Gpnmb, whose roles in the deterioration of obesity-associated adipose tissue are poorly understood. In conclusion, the genes identified here provide new therapeutic opportunities for prevention and treatment of diet-induced obesity.