Human burst-forming units-erythroid need direct interaction with stem cell factor for further development.
Human burst-forming units-erythroid need direct interaction with stem cell factor for further development.
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DOI：
10.1182/blood.v78.10.2493.bloodjournal78102493
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发表时间：
1991-11
期刊：
影响因子：
20.3
通讯作者：
C. Dai;S. Krantz;K. Zsebo
中科院分区：
文献类型：
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作者：
C. Dai;S. Krantz;K. Zsebo
为了了解未成熟红细胞祖细胞（burst-forming units-erythroid, BFU-E）早期生长发育的调控因素，需要高度纯化的靶细胞和无血清培养基。当高度纯化的人血液BFU-E在无血清培养基中培养时，BFU-E即使添加重组人白细胞介素-3 （il -3）也不能生长，il -3是这些细胞所必需的。然而，加入支持生殖细胞和多能干细胞生长的重组人干细胞因子（rSCF），刺激BFU-E在无血清和含血清培养基中同样生长良好。限制性稀释研究表明，rSCF直接作用于不需要辅助细胞生长的BFU-E。此外，在培养的最初7天，rSCF对于BFU-E的发育是必需的，直到这些细胞达到晚期祖细胞阶段，即集落形成单位-红系（CFU-E）。这些研究表明，早期红细胞生成依赖于SCF的直接作用，SCF不仅影响早期干细胞，而且对于红细胞祖细胞的进一步发育是持续必要的，直到CFU-E成熟阶段。
To understand the factors that regulate the early growth and development of immature erythroid progenitor cells, the burst-forming units-erythroid (BFU-E), it is necessary to have both highly purified target cells and a medium free of serum. When highly purified human blood BFU-E were cultured in a serum-free medium adequate for the growth of later erythroid progenitors, BFU-E would not grow even with the addition of recombinant human interleukin-3 (rIL-3), known to be essential for these cells. However, the addition of recombinant human stem cell factor (rSCF), which supports germ cell and pluripotential stem cell growth, stimulated BFU-E to grow equally well in serum-free as in serum-containing medium. Limiting dilution studies showed that rSCF acts directly on the BFU-E that do not require accessory cells for growth. Furthermore, rSCF was necessary for BFU-E development during the initial 7 days of culture, until these cells reached the stage of the late progenitors, the colony-forming units-erythroid (CFU-E). These studies indicate that early erythropoiesis is dependent on the direct action of SCF that not only affects early stem cells but is continually necessary for the further development of committed erythroid progenitor cells until the CFU-E stage of maturation.