Pod-1, a mesoderm-specific basic-helix-loop-helix protein expressed in mesenchymal and glomerular epithelial cells in the developing kidney
Pod-1, a mesoderm-specific basic-helix-loop-helix protein expressed in mesenchymal and glomerular epithelial cells in the developing kidney
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DOI：
10.1016/s0925-4773(97)00201-3
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发表时间：
1998-02-01
影响因子：
2.6
通讯作者：
Igarashi, P
中科院分区：
文献类型：
--
作者：
Quaggin, SE;Vanden Heuvel, GB;Igarashi, P
碱性螺旋环螺旋蛋白(BHLH)是一种转录调控蛋白，控制着多种组织中细胞命运的决定和分化。我们已经鉴定出一种新的bHLH蛋白，命名为Pod-1，属于最近描述的bHLH蛋白亚家族，在中胚层组织的胚胎发育中具有重要作用。在成年人和小鼠中，Pod-1在肾脏、肺和心脏中表达最高。在发育中的小鼠胚胎中，Pod-1选择性地表达于肾、肺、肠和胰腺上皮-间充质相互作用部位的间充质细胞。POD-1在肾脏内脏肾小球上皮细胞(足细胞)中也有表达，其表达与足细胞分化的开始相一致。反义寡核苷酸可抑制胚胎肾组织中Pod-1的表达。抑制Pod-1的表达导致输尿管芽周围间充质细胞凝聚减少，输尿管分支减少40%。POD-1是在发育中的肾脏中发现的第一个组织限制性碱性螺旋-环-螺旋蛋白，它可能在形态发生事件的调节中发挥作用。(C)1998年爱思唯尔爱尔兰科学有限公司。
Basic-helix-loop-helix (bHLH) proteins are transcriptional regulatory proteins that govern cell fate determination and differentiation in a variety of tissues. We have identified a novel bHLH protein, named Pod-1, that belongs to a recently-described subfamily of bHLH proteins that have essential roles in the embryonic development of mesodermal tissues. In the adult human and mouse, Pod-1 was most highly expressed in the kidney, lung and heart. In developing mouse embryos, Pod-1 was selectively expressed in mesenchymal cells at sites of epithelial-mesenchymal interaction in the kidney, lung, intestine and pancreas. Pod-1 was also expressed in visceral glomerular epithelial cells (podocytes) in the kidney, and its expression coincided with the onset of podocyte differentiation. The expression of Pod-1 in embryonic kidney explants was inhibited using antisense oligonucleotides. Inhibition of Pod-1 expression resulted in decreased mesenchymal cell condensation around the ureteric bud and a 40% decrease in ureteric branching. Pod-1 is the first tissue-restricted basic-helix-loop-helix protein that has been identified in the developing kidney where it may play a role in the regulation of morphogenetic events. (C) 1998 Elsevier Science Ireland Ltd.