Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females.
Deletion mapping of the testis determining locus with DNA probes in 46,XX males and in 46,XY and 46,X,dic(Y) females.
复制标题
使用 DNA 探针对 46,XX 男性和 46,XY 和 46,X,dic(Y) 女性的睾丸确定基因座进行删除作图。
DOI：
10.1093/nar/14.16.6489
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发表时间：
1986
影响因子：
14.9
通讯作者：
Latt,SA
中科院分区：
文献类型：
--
作者：
Müller,U;Donlon,T;Schmid,M;Fitch,N;Richer,CL;Lalande,M;Latt,SA
从流式分选的人Y染色体构建的重组噬菌体DNA文库中分离出11个与一个或多个46，XX男性DNA杂交的Y特异性DNA探针。两个探针与来自十一个中的九个的DNA杂交，即这些46，XX雄性中的>80%。探针在46，XX雄性和46，X，dic（Y）雌性中杂交的相对频率，以及原位杂交数据，允许将探针定位在与推定的睾丸决定基因座相关的Yp上。这些探针中的几个在46，XY女性中也不存在，进一步完善了用于在Yp上排序探针的模型。一个XX男性的DNA与来自Yp的探针和来自近端Yq的探针（不包括中心周围区域）杂交。这表明，复杂的易位可能发生在46，XX男性的DNA中，不仅涉及部分Yp，而且涉及部分yq
Eleven Y-specific DNA probes hybridizing with DNA from one or more 46, XX males were isolated from a recombinant phage DNA library constructed from flow sorted human Y chromosomes. Two probes hybridized with DNA from nine out of eleven, i.e. >80% of these 46, XX males. The relative frequency of hybridization of the probes in the 46, XX males and in a 46, X,dic(Y) female, together with in situ hybridization data, allowed mapping of the probes on Yp in relation to a putative testis determining locus. Several of those probes were also absent in a 46,XY female, further refining a model for ordering the probes on Yp. The DNA of one XX male hybridized both with probes from Yp and probes from proximal Yq (excluding the pericentral region). This suggests that complex translocations may occur into the DNA of 46, XX males that involve not only parts of Yp but also parts of yq