Study of molecular karyotypes in Amoeboaphelidium protococcarum, the endotrophic parasite of chlorophycean alga Scenedesmus
Study of molecular karyotypes in Amoeboaphelidium protococcarum, the endotrophic parasite of chlorophycean alga Scenedesmus
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DOI：
10.1007/s002849900155
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发表时间：
1997-02-01
影响因子：
2.6
通讯作者：
Nasonova, E
中科院分区：
文献类型：
--
作者：
Pinevich, A;Gromov, B;Nasonova, E
基于脉冲场凝胶电泳（PFGE）技术，测定了原球变形虫（绿藻内养寄生虫，结合了原生动物和真菌型特征）的分子核型。菌株X1、X5和X31的分子核型，其宿主范围不同，分别起源于欧亚西部、中部和东部，显示了属内多态性——7至13条chDNA条带——估计分子大小为0.3-2.2 Mb，并且确定基因组大小在7.4至10.2 Mb之间。原球菌的分子核型模式与原生动物不同。一方面，X1 和 X5 之间分子核型的低级区别，另一方面，X1/X5 和 X31 之间的高级区别，使得将 X31 置于单独的分类单元中成为可能。
On the basis of the pulsed field gel electrophoresis (PFGE) technique, molecular karyotypes in Amoeboaphelidium protococcarum (the endotrophic parasite of chlorophycean algae, which combines protozoon- and fungus-type characters) were determined. Molecular karyotypes in the strains X1, X5, and X31, which differ in the host range and originate from Western, Central, and Eastern Euro-Asia respectively, demonstrate the intrageneric polymorphism-from 7 to 13 chDNA bands-with the estimated molecular size of 0.3-2.2 Mb were resolved, and the genome size was determined to be between 7.4 and 10.2 Mb. The molecular karyotype pattern in A. protococcarum is different from that in Protozoa. The low-grade distinction in molecular karyotypes between X1 and X5, on one hand, and high-grade distinction between X1/X5 and X31, on the other hand, makes it possible to place X31 in a separate taxon.