Identification of iron-regulated genes of Bifidobacterium breve UCC2003 as a basis for controlled gene expression
Identification of iron-regulated genes of Bifidobacterium breve UCC2003 as a basis for controlled gene expression
复制标题
DOI：
10.4161/bbug.18985
复制
发表时间：
2012-05-01
期刊：
影响因子：
4.9
通讯作者：
van Sinderen, Douwe
中科院分区：
文献类型：
--
作者：
Cronin, Michelle;Zomer, Aldert;van Sinderen, Douwe
Iron is an essential growth factor for virtually all organisms. However, iron is not readily available in most environments and microorganisms have evolved specialized mechanisms, such as the use of siderophores and high-affinity transport systems, to acquire iron when confronted with iron-limiting conditions. In general these systems are tightly regulated to prevent iron-induced toxicity and because they are quite costly to the microbe. Because of this tight regulation we chose to explore the response of Bifidobacterium breve UCC2003 to iron limitation. Through microarray and complementation analyses we identified and characterized a presumed ferrous iron uptake system, encoded by bfeUOB, from B. breve UCC2003 and exploited its regulated transcription to develop an inducible expression system for use in bifidobacteria.
