Prolongation of murine skin allograft survival by the systemic effects of 8-methoxypsoralen and long-wave ultraviolet radiation (PUVA).
Prolongation of murine skin allograft survival by the systemic effects of 8-methoxypsoralen and long-wave ultraviolet radiation (PUVA).
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8-甲氧基补骨脂素和长波紫外线辐射 (PUVA) 的全身作用可延长小鼠皮肤同种异体移植物的存活率。
DOI：
10.1111/1523-1747.ep12469766
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Parrish,JA
中科院分区：
文献类型：
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作者：
Granstein,RD;Smith,L;Parrish,JA
对一组带有皮肤同种异体移植物的小鼠全身给予光敏药物8-甲氧基补骨脂素，然后在远离同种异体移植物的部位每天暴露于长波紫外线辐射（UVA，320-400 nm）（PUVA）14天，显著增加了同种异体移植物的存活时间。这种效应在主要组织相容性复合体不同的供体-受体组合和仅在次要组织相容性位点不同的供体-受体组合中均可见。单独使用8-甲氧基补骨脂素或长波紫外线辐射治疗在延长同种异体移植物存活方面无效，中波紫外线辐射（UVB，280-320 nm）的剂量比PUVA方案产生更大的炎症。同种异体移植，PUVA治疗的动物也表现出降低同种异体抗原反应性对供体品系脾细胞在治疗期间的细胞毒性试验。在小鼠系统中，皮肤的同种异体移植物具有高度免疫原性，并且通常比器官同种异体移植物更快地被排斥;因此PUVA治疗似乎对延长同种异体移植物存活发挥有效作用。该效应的全身性质以及PUVA的不良副作用主要限于皮肤的事实表明，PUVA可能在临床器官移植管理中发挥作用。
Systemic administration of the photoactive drug 8-methoxypsoralen to a group of mice bearing cutaneous allografts, followed by exposure to long-wave ultraviolet radiation (UVA, 320–400 nm) (PUVA) daily for 14 days at a site distant from the allograft, significantly increased the survival time of the allografts. This effect was seen both in donor-recipient combinations that differ at the major histocompatibility complex and in those differing only at minor histocompatibility loci. Treatment with 8-methoxypsoralen or long-wave UV radiation alone was ineffective in prolonging allograft survival, as were doses of mid-wave UV radiation (UVB, 280–320 nm) that produced greater inflammation than the PUVA protocol. Allografted, PUVA-treated animals also demonstrated decreased alloantigen reactivity against donor-strain spleen cells during the period of treatment by cytotoxicity assays. Allografts of skin in the murine system are highly immunogenic and are generally rejected faster than organ allografts; thus PUVA treatment appears to exert a potent effect on prolonging allograft survival. The systemic nature of the effect and the fact that adverse side effects from PUVA are largely limited to the skin suggest that PUVA might have a role in clinical organ transplantation management.