Morphological and molecular characterisation, and phylogenetic position of X. browni sp. n., X. penevi sp. n. and two known species of Xiphinema americanum-group (Nematoda, Longidoridae).
Morphological and molecular characterisation, and phylogenetic position of X. browni sp. n., X. penevi sp. n. and two known species of Xiphinema americanum-group (Nematoda, Longidoridae).
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X. Browni sp。的形态和分子表征以及系统发育位置。 n。，X。Penevisp。 n。以及两种已知的Xiphinema Americanum-Group（Nematoda，Longidoridae）。
DOI：
10.3897/zookeys.574.8037
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发表时间：
2016
期刊：
影响因子：
1.3
通讯作者：
Kumari S
中科院分区：
文献类型：
--
作者：
Lazarova S;Peneva V;Kumari S
利用核糖体（18 S，ITS 1，ITS 2，28 SrDNA的D2-D3扩增片段）和线粒体（部分cox 1和nad 4）DNA标记，对来自欧洲和摩洛哥的美洲剑线虫（Xiphinema americpudi group）的几个种群进行了研究，发现了两个隐种：褐剑线虫Xiphinema browni sp. n.（以前报道为Xiphinema pachtaicum）和Xiphinema penevi sp. n.被揭露了描述了种，说明和它们的系统发育关系进行了讨论。第一个种与副剑线虫最为相似，是剑线虫种复合体的一员。利用18 S、D2-D3、28 S rDNA和cox 1 3个分子标记进行系统发育分析，结果表明：Penevi Xiphinema sp. n.是Xiphinema pachtaicum亚群的一部分，与Xiphinema incertum、Xiphinema pachtaicum、Xiphinema parahachydermum、Xiphinema plesiopachtaicum、Xiphinema astaregiense和Xiphinema pachydermum关系密切。此外，一个单独的“剑线虫相似亚群”，在剑线虫pachtaicum亚群之外，迄今为止只包括孤雌生殖种剑线虫Xiphinema pachtaicum，剑线虫Xiphinema paraicum，剑线虫Xiphinema browni sp. n。很可能形成了剑线虫设计并使用新的引物扩增和测序线粒体nad 4基因的一部分。
Using ribosomal (18S, ITS1, ITS2, D2-D3 expansion segments of 28S rDNA) and mitochondrial (partial cox1 and nad4) DNA markers in a study of several populations of Xiphinema americanum-group from Europe and Morocco, two cryptic species Xiphinema browni sp. n. (formerly reported as Xiphinema pachtaicum) and Xiphinema penevi sp. n. were revealed. The species are described, illustrated and their phylogenetic relationships discussed. The first species is most similar to Xiphinema parasimile and is a member of Xiphinema simile species complex. The phylogenetic reconstructions inferred from three molecular markers (18S, D2-D3 28S rDNA and cox1) showed that Xiphinema penevi sp. n. is part of Xiphinema pachtaicum-subgroup and is closely related to Xiphinema incertum, Xiphinema pachtaicum, Xiphinema parapachydermum, Xiphinema plesiopachtaicum, Xiphinema astaregiense and Xiphinema pachydermum. Also, a separate “Xiphinema simile-subgroup”, outside the Xiphinema pachtaicum-subgroup and so far consisting only of the parthenogenetic species Xiphinema simile, Xiphinema parasimile, Xiphinema browni sp. n. and probably Xiphinema vallense was formed. New primers for amplification and sequencing of part of the nad4 mitochondrial gene were designed and used.