Collagen-Hydroxyapatite Scaffolds Induce Human Adipose Derived Stem Cells Osteogenic Differentiation In Vitro.
Collagen-Hydroxyapatite Scaffolds Induce Human Adipose Derived Stem Cells Osteogenic Differentiation In Vitro.
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胶原 - 羟基磷灰石支架在体外诱导人脂肪衍生的干细胞成骨分化。
DOI：
10.1371/journal.pone.0151181
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Forte S
中科院分区：
文献类型：
--
作者：
Calabrese G;Giuffrida R;Fabbi C;Figallo E;Lo Furno D;Gulino R;Colarossi C;Fullone F;Giuffrida R;Parenti R;Memeo L;Forte S
间充质干细胞（MSC）在调节正常骨骼稳态，以及在损伤的情况下，在骨愈合和重建骨骼完整性中起着至关重要的作用。最近的科学文献集中于骨再生模型的开发，其中MSC与能够指导MSC成骨的仿生三维支架组合。在这项工作中，从脂肪组织中分离的人MSC（hADSC）的成骨潜力已经在体外与胶原/Mg掺杂的羟基磷灰石支架组合进行了评估。茜素红S染色和基因表达谱分析表明，在特异性分化诱导培养基中培养的hADSC具有高的成骨潜能。与胶原/羟基磷灰石支架复合后，hADSCs即使在缺乏特异性诱导因子的情况下也能分化为成熟的成骨细胞;然而，这些因子的补充显著地加速了成骨过程，如通过前成骨细胞和成熟成骨细胞阶段的特异性标志物（如osterix、骨桥蛋白、（也称为骨唾液蛋白I）、骨钙素和细胞外基质成熟和矿化阶段的特异性标志物，如ALPL和骨粘连蛋白。因此，目前的工作表明，支架本身能够诱导hADSC分化，而骨诱导因子的添加产生成骨过程的显着加速。这一观察结果使得我们的模型在再生医学领域治疗骨缺损的应用具有潜在的意义。
Mesenchymal stem cells (MSCs) play a crucial role in regulating normal skeletal homeostasis and, in case of injury, in bone healing and reestablishment of skeletal integrity. Recent scientific literature is focused on the development of bone regeneration models where MSCs are combined with biomimetic three-dimensional scaffolds able to direct MSC osteogenesis. In this work the osteogenic potential of human MSCs isolated from adipose tissue (hADSCs) has been evaluated in vitro in combination with collagen/Mg doped hydroxyapatite scaffolds. Results demonstrate the high osteogenic potential of hADSCs when cultured in specific differentiation induction medium, as revealed by the Alizarin Red S staining and gene expression profile analysis. In combination with collagen/hydroxyapatite scaffold, hADSCs differentiate into mature osteoblasts even in the absence of specific inducing factors; nevertheless, the supplement of the factors markedly accelerates the osteogenic process, as confirmed by the expression of specific markers of pre-osteoblast and mature osteoblast stages, such as osterix, osteopontin (also known as bone sialoprotein I), osteocalcin and specific markers of extracellular matrix maturation and mineralization stages, such as ALPL and osteonectin. Hence, the present work demonstrates that the scaffold per se is able to induce hADSCs differentiation, while the addition of osteo-inductive factors produces a significant acceleration of the osteogenic process. This observation makes the use of our model potentially interesting in the field of regenerative medicine for the treatment of bone defects.