Retention of ionic and non-ionic catechols in capillary zone electrophoresis with micellar solutions.
Retention of ionic and non-ionic catechols in capillary zone electrophoresis with micellar solutions.
复制标题
胶束溶液毛细管区带电泳中离子和非离子儿茶酚的保留。
DOI：
10.1016/s0021-9673(01)83873-5
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Ewing,AG
中科院分区：
文献类型：
--
作者：
Wallingford,RA;Ewing,AG
胶束溶液在毛细管区带电泳中的应用主要局限于非离子溶质的分离，而其对阳离子溶质的适用性尚未得到探索。我们已经发现，在磷酸盐缓冲液中使用十二烷基硫酸钠胶束允许在几个阳离子和非离子儿茶酚的选择性方面的巨大收益，超过可以用正常的毛细管区带电泳获得的。邻苯二酚与硼酸的络合作用改变了溶质的净电荷，并改变了分配行为，从而产生了足够的选择性，缩短了分析时间。虽然溶质与胶束相的阳离子物种的相互作用的机制还没有决定性的已知，证据支持存在的离子配对平衡，同时伴随着胶束增溶。
The use of micellar solutions in capillary zone electrophoresis has been primarily relegated to separations of non-ionic solutes, while its applicability to cationic species has been unexplored. We have found that the use of sodium dodecyl sulfate micelles in phosphate buffer allows for tremendous gains in selectivity for several cationic and non-ionic catechols over what can be obtained with normal capillary zone electrophoresis. Complexation of catechols with boric acid alters the net charge on the solutes and changes the partitioning behavior to produce adequate selectivity with improved analysis times. Although the mechanisms of solute interaction with the micellar phase for the cationic species are not decisively known, evidence is presented supporting the existence of ion-pairing equilibria simultaneously accompanied by micellar solubilization.