In situ tissue engineering of periodontal tissues by seeding with periodontal ligament-derived cells
In situ tissue engineering of periodontal tissues by seeding with periodontal ligament-derived cells
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DOI：
10.1089/107632704323061898
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发表时间：
2004-03-01
影响因子：
--
通讯作者：
Shimizu, Y
中科院分区：
文献类型：
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作者：
Nakahara, T;Nakamura, T;Shimizu, Y
研究了采用自体牙周膜衍生细胞进行细胞治疗的原位组织工程方法的可行性。牙周膜细胞取自六只比格犬。在上颌尖牙边缘牙槽嵴顶端 6 毫米的位置两侧创建牙周开窗缺损 (6x4 毫米)。在植入前将碱性磷酸酶阳性牙周膜细胞（3x10(5) 细胞）接种到胶原海绵支架上。一个缺陷被细胞支架结构填充，另一个缺陷留空作为对照。手术后4周处死所有动物，并对标本进行组织形态计量学评估。所有组织形态计量数据均通过 Bonferroni 多重比较检验进行三向方差分析。对照侧根尖组织的再生速度快于冠状组织和孤立组织（根尖 > 冠状 > 孤立组织；p
The feasibility of an in situ tissue-engineering method employing cell-based therapy with autologous periodontal ligament-derived cells was investigated. Periodontal ligament cells were obtained from six beagle dogs. Periodontal fenestration defects (6x4 mm) were created bilaterally at a location 6 mm apical to the marginal alveolar crest in the maxillary canines. Alkaline phosphatase-positive periodontal ligament cells (3x10(5) cells) were seeded onto a collagen sponge scaffold just before implantation. One defect was filled with the cell-scaffold construct, and another was left empty as the control. All animals were killed 4 weeks after surgery, and specimens were evaluated histomorphometrically. All the histomorphometrical data were analyzed by three-way analysis of variance with the Bonferroni multiple comparisons test. Regeneration of apical tissue was faster than that of coronal and isolated tissues on the control side (apical > coronal > isolated; p