Biocatalytic oxidase: Batch to continuous
Biocatalytic oxidase: Batch to continuous
复制标题
DOI：
10.1016/j.cherd.2012.01.018
复制
发表时间：
2012-06-01
影响因子：
3.9
通讯作者：
Archer, Ian
中科院分区：
文献类型：
--
作者：
Jones, Edward;McClean, Kay;Archer, Ian
采用更有效的发展战略和制造技术对于生物制造业未来的成功至关重要。生物催化过程的连续操作有可能提供许多优于已建立的批处理方法的优点。存在改进工艺控制、易于放大、使生产中断最小化、减小反应器尺寸和经济地使用生物催化剂的机会。Coflore的设计采用了专利混合技术，当反应器主体受到横向振动时，每个反应阶段内的自由移动搅拌器促进混合。多个离散（互连）反应池提供良好的混合和活塞流，并且该设计允许使用浆料和处理气体/液体混合物。Coflore搅拌管式反应器（ATR）是一种工业管式反应器，用于均相和两相流体。采用与实验室规模的Coflore ACR相同的混合原理，它使用横向运动来产生混合和阶段分离，以防止返混。我们描述了这些连续活塞流反应器在生物过程开发中的应用，从简单的实验室规模的批处理过程开始，通过台式活塞流反应器（ACR），再到多升生产规模的搅拌管式反应器（ATR）。该报告将比较在间歇和连续条件下运行的D-氨基酸氧化酶（DAAO）催化的氧化反应的结果，并将说明ACR和ATR反应器的应用如何促进工艺开发。(C)2012化学工程师学会。Elsevier B. V.出版，保留所有权利。
The adoption of more efficient development strategies and manufacturing techniques will be essential for future success in the bio manufacturing sectors. Continuous operation of biocatalytic processes has the potential to offer many advantages over established batch process methodologies. There exist opportunities for improved process control; ease of scale up; minimizing of interruptions in production; reducing reactor size; and economic use of biocatalysts.The Coflore (TM) Agitated Cell Reactor (ACR) is a dynamically mixed plug flow reactor. The Coflore design employs a patented mixing technique where free moving agitators within each reaction stage promote mixing when the reactor body is subjected to lateral shaking. Multiple discrete (interlinked) reaction cells give good mixing and plug flow, and the design permits the use of slurries and handling of gas/liquid mixtures. The Coflore Agitated Tube Reactor (ATR) is an industrial tube flow reactor for homogenous and two phase fluids. Employing the same mixing principle as the lab scale Coflore ACR, it uses lateral movement to generate mixing and stage separation to prevent back mixing.We describe the application of these continuous plug flow reactors for bioprocess development starting from simple lab scale batch processes; through benchtop plug flow reactors (ACRs); and on to the multi-litre production scale agitated tube reactor (ATR). The presentation will compare the results of an oxidation reaction catalysed by D-amino acid oxidase (DAAO) operated under batch and continuous conditions, and will illustrate how application of the ACR and ATR reactors can facilitate process development. (C) 2012 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.