MUC4 expression increases progressively in pancreatic intraepithelial neoplasia.
MUC4 expression increases progressively in pancreatic intraepithelial neoplasia.
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DOI：
10.1309/7y7n-m1wm-r0yk-m2va
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发表时间：
2002-05
影响因子：
3.5
通讯作者：
M. Swartz;S. Batra;G. C. Varshney;M. Hollingsworth;C. Yeo;J. Cameron;R. Wilentz;R. Hruban;P. Argani
中科院分区：
文献类型：
--
作者：
M. Swartz;S. Batra;G. C. Varshney;M. Hollingsworth;C. Yeo;J. Cameron;R. Wilentz;R. Hruban;P. Argani
胰腺癌被认为是由组织学上可识别的导管内病变（称为胰腺上皮内瘤形成（PanIN））发展而来，这些病变经历了一系列结构、细胞学和遗传学变化，其进展模式与结肠中的腺瘤-癌序列相似。无粘蛋白MUC 4与侵袭性胰腺癌有关。MUC 4的表达在正常胰腺中在RNA水平上是不可检测的，但在侵袭性胰腺癌中在高水平上是可检测的。我们通过使用一种新的MUC 4单克隆抗体对一系列71个PanIN病变进行免疫化学研究，记录了MUC 4在PanIN中的表达模式。30个PanIN-1病变中的5个（17%），28个PanIN-2病变中的10个（36%），13个PanIN-3病变中的11个（85%），以及28个浸润性腺癌中的25个（89%）用研究中使用的MUC 4抗体标记。此外，一些非肿瘤性病变也被MUC 4抗体标记，包括慢性胰腺炎中的反应性导管、充满坏死分泌物的萎缩导管和显示鳞状化生的导管。我们的数据有助于建立胰腺肿瘤前体中MUC 4表达的模式，并进一步支持胰腺癌的进展模型。
Pancreatic adenocarcinoma is believed to develop from histologically identifiable intraductal lesions known as pancreatic intraepithelial neoplasias (PanINs) that undergo a series of architectural, cytologic, and genetic changes, a progression model similar to the adenoma-carcinoma sequence in the colon. The apomucin MUC4 has been implicated in invasive pancreatic adenocarcinoma. MUC4 expression is not detectable at the RNA level in normal pancreas but is detectable at high levels in invasive pancreatic adenocarcinoma. We documented the pattern of expression of MUC4 in PanINs by studying a series of 71 PanIN lesions immunohistochemically using a new monoclonal antibody to MUC4. Five (17%) of 30 PanIN-1 lesions, 10 (36%) of 28 PanIN-2 lesions, 11 (85%) of 13 PanIN-3 lesions, and 25 (89%) of 28 invasive adenocarcinomas labeled with the MUC4 antibody used in the study. In addition, afew nonneoplastic lesions labeled with the MUC4 antibody, including reactive ducts in chronic pancreatitis, atrophic ducts filled with inspissated secretions, and ducts showing squamous metaplasia. Our data help establish the patterns of MUC4 expression in neoplastic precursors in the pancreas and add further support to the progression model for pancreatic adenocarcinoma.