CHARACTERIZATION OF THE ENZYMATIC-HYDROLYSIS OF ACETATE FROM ALKYLACETYLGLYCEROLS IN THE DENOVO PATHWAY OF PAF BIOSYNTHESIS
CHARACTERIZATION OF THE ENZYMATIC-HYDROLYSIS OF ACETATE FROM ALKYLACETYLGLYCEROLS IN THE DENOVO PATHWAY OF PAF BIOSYNTHESIS
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DOI：
10.1016/0005-2760(90)90001-e
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发表时间：
1990-02-06
期刊：
影响因子：
--
通讯作者：
SNYDER, F
中科院分区：
文献类型：
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作者：
BLANK, ML;SMITH, ZL;SNYDER, F
本报告描述了负责从 1-烷基-2-乙酰基-sn-甘油水解乙酸盐的酶活性的部分特征，1-烷基-2-乙酰基-sn-甘油是艾利希腹水细胞从头合成 PAF（血小板激活因子或 1-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱）的直接前体。这种中性脂质的最高乙酰水解酶活性与艾利希腹水细胞的膜部分相关（> 总活性的 90%）；仅进行最低限度的活动（
This report describes the partial characterization of the enzymatic activity responsible for the hydrolysis of acetate from 1-alkyl-2-acetyl-sn-glycerol, the immediate precursor in the de novo synthesis of PAF (platelet-activating factor or 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine) by Ehrlich ascites cells. The highest acetylhydrolase activity for this neutral lipid was associated with the membrane fractions from Ehrlich ascites cells (> 90% of total activity); only a minimal level of activity (