Prolyl-hydroxyproline dipeptide in non-hydrolyzed morning urine and its value in postmenopausal osteoporosis
Prolyl-hydroxyproline dipeptide in non-hydrolyzed morning urine and its value in postmenopausal osteoporosis
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DOI：
10.1515/cclm.2008.259
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发表时间：
2008-10-01
影响因子：
6.8
通讯作者：
Simek, Petr
中科院分区：
文献类型：
--
作者：
Husek, Petr;Svagera, Zdenek;Simek, Petr
背景资料：由于脯氨酰-4-羟脯氨酸二肽在非水解尿和水解尿中的4-羟脯氨酸的水平之间的高度相关性，我们研究是否二肽可能作为一个有价值的标志物骨turning.Methods：基于光密度测量，68绝经后妇女被分为非骨病，骨质疏松和骨质疏松症的受试者。采用液相色谱法测定晨尿中的二肽和尿吸收标志物，采用免疫分析法测定晨尿中的尿吸收标志物。连同分析的基础水平，饮食相关的变化和愈合效果的每年阿仑膦酸钠therapeutic assessed.Results：浓度水平在控制和tumorotic主题显着不同;接收器操作特性产生的灵敏度为0.743，特异性为0.908，曲线下面积为0.903，和截止值为10.2 mmol/mmol的肌酐。斯皮尔曼等级相关显示二肽与骨钙素之间的配对系数最高。饮食相关的变化没有发现。治疗后，一个显着的下降已经发生在三个月内，而与其他的再吸收标记物，直到6 months.Conclusions：在非水解尿中的二肽测定的容易程度超过了羟脯氨酸，和结果目前的化合物作为一个真实的竞争，其他常见的评估标志物在骨病。
Background: Owing to the high correlation between the level of prolyl-4-hydroxyproline dipeptide in non-hydrolyzed urine and that of 4-hydroxyproline in hydrolyzed urine, we examined whether the dipeptide might function as a valuable marker of bone turnover.Methods: Based on densitometric measurements, 68 postmenopausal women were divided into groups of non-osteopathic, osteopenic and osteoporotic subjects. The dipeptide and current urinary resorption markers were assayed in morning urine, the former using liquid chromatography, the others plus serum formation markers by means of immunoassay procedures. Together with the assay of basal levels, diet-related changes and healing effect of yearly alendronate therapy were assessed.Results: Concentration levels in controls and osteoporotic subjects differed significantly; receiver operating characteristics yielded sensitivity of 0.743, specificity of 0.908, area under curve of 0.903, and cutoff of 10.2 mmol/mmol of creatinine. Spearman rank correlation showed the highest pair coefficient between the dipeptide and osteocalcin. Diet-related changes were not found. Following therapy, a significant decline occurred already within a trimester, whilst with the other resorption markers not until 6 months.Conclusions: The ease of the dipeptide assay in non-hydrolyzed urine surpasses that of hydroxyproline, and the results present the compound as a real competition to other commonly assessed markers in osteopathies.