The influence of alumina and ultra-high molecular weight polyethylene particles on osteoblast-osteoclast cooperation
The influence of alumina and ultra-high molecular weight polyethylene particles on osteoblast-osteoclast cooperation
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DOI：
10.1016/j.biomaterials.2003.10.100
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发表时间：
2004-08-01
期刊：
影响因子：
14
通讯作者：
Giunti, A
中科院分区：
文献类型：
--
作者：
Granchi, D;Ciapetti, G;Giunti, A
颗粒诱导的巨噬细胞活化（主要由UHMWPE磨损引起）已被认为是导致假体周围骨吸收的生物学机制，这是全髋关节置换术（THR）松动的原因。陶瓷对陶瓷植入物已被提倡作为减少磨损产品的一种手段。许多研究调查了氧化铝（Al 2 O3）颗粒对单核细胞/巨噬细胞的影响，但关于其参与骨转换的信息有限。进行体外模型以研究Al 2 O3和UHMWPE颗粒如何影响成骨细胞-破骨细胞相互作用：人成骨细胞（HOB）来自松质骨，而破骨细胞来自健康供体的外周血单核细胞（PBMC）。在颗粒激发的HOB（HOB-CM）的培养基中检测IL 6、TNF α、GM-CSF和其它作用于骨转换的因子，即“NF κ B受体激活剂”配体（RANKL）和骨保护素（OPG）的量。Al 2 O3和UHMWPE颗粒不影响细胞活力或TNF和GM-CSF释放，而IL 6释放的增加似乎取决于颗粒浓度。UHMWPE增加了HOB中RANKL的释放，而OPG和OPG与RANKL的比值显著抑制。通过成骨细胞/单核细胞合作检测HOB-CM促进破骨细胞生成的能力：培养7天后，UHMWPE HOB-CM诱导大量多核TRAP阳性巨细胞，并显著降低上清液中IL 6、GM-CSF和RANKL的量。关于破骨细胞生成的诱导作用，我们的研究结果表明，氧化铝磨屑是不太活跃。(C)2003 Elsevier Ltd.保留所有权利。
Particle-induced macrophage activation, mainly by UHMWPE wear, has been recognized as the biological mechanism leading to periprosthetic bone resorption, which is responsible for the loosening of the total hip replacements (THR). Ceramic-on-ceramic implants have been advocated as a means of reducing wear products. Many studies investigated the effect of alumina (Al2O3) particles on monocytes/macrophages, but only limited information are available on their participation to bone turnover. An in vitro model was performed to investigate how Al2O3 and UHMWPE particles may influence the osteoblast-osteoclast interaction: human osteoblasts (HOB) were obtained from trabecular bone, while osteoclasts were derived from peripheral blood mononuclear cells (PBMC) of healthy donors. The amount of IL6, TNFalpha, GM-CSF, and other factors acting on the bone turnover, i.e. the 'receptor activator of NFkB' ligand (RANKL) and osteoprotegerin (OPG), was detected in culture medium of particle-challenged HOB (HOB-CM). The Al2O3 and UHMWPE particles did not affect either cell viability or TNF and GM-CSF release, while the increase in IL6 release seemed to be dependent on the particle concentration. UHMWPE increased the release of RANKL from HOB, while OPG and OPG-to-RANKL ratio were significantly inhibited. The ability of HOB-CM to promote osteoclastogenesis was tested via osteoblast/monocyte cooperation: after seven days of culture UHMWPE HOB-CM induced a large amount of multinucleated TRAP-positive giant cells, as well as significantly reduced the amount of IL6, GM-CSF and RANKL in the supernatant. With regard to the inductive effect on the osteoclastogenesis, our results show that the Al2O3 wear debris are less active. (C) 2003 Elsevier Ltd. All rights reserved.