Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus
Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus
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DOI：
10.1016/s0140-6736(97)11212-0
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发表时间：
1998-02-14
期刊：
影响因子：
168.9
通讯作者：
Webster, RG
中科院分区：
文献类型：
--
作者：
Claas, ECJ;Osterhaus, ADME;Webster, RG
背景1997年5月，香港一名3岁男童入院，随后死于流感肺炎、急性呼吸窘迫综合征、Reye综合征、多器官衰竭和弥散性血管内凝血。从该男童的气管吸出物中分离出甲型流感H5 N1病毒。在此事件发生前，香港有三个鸡场爆发高死亡率的禽流感，方法采用血凝抑制法和神经氨酸酶法对从该患儿分离的H5 N1亚型流感病毒与从禽流感暴发流行中分离的H5 N1亚型流感病毒进行抗原性和分子生物学比较。结果通过血凝抑制试验观察到病毒抗原反应性的差异，然而，所有基因片段的核苷酸序列分析显示，人类病毒A/Hong Kong/156/97与禽流感病毒A/chicken/Hong Kong/258/97.解释虽然尚未确定患病儿童与受感染鸡之间的直接接触，但我们的研究结果表明，病毒从受感染鸡传播给儿童，而没有中间哺乳动物宿主作为作用容器”。这一事件说明了全球流感监测工作的加强。
Background In May, 1997, a 3-year-old boy in Hong Kong was admitted to the hospital and subsequently died from influenza pneumonia, acute respiratory distress syndrome, Reye's syndrome, multiorgan failure, and disseminated intravascular coagulation. An influenza A H5N1 virus was isolated from a tracheal aspirate of the boy. Preceding this incident, avian influenza outbreaks of high mortality were reported from three chicken farms in Hong Kong, and the virus involved was also found to be of the H5 subtype.Methods We carried out an antigenic and molecular comparison of the influenza A H5N1 virus isolated from the boy with one of the viruses isolated from outbreaks of avian influenza by haemagglutination-inhibition and neuraminidase-inhibition assays and nucleotide sequence analysis.Findings Differences were observed in the antigenic reactivities of the viruses by the haemagglutination-inhibition assay, However, nucleotide sequence analysis of all gene segments revealed that the human virus A/Hong Kong/156/97 was genetically closely related to the avian A/chicken/Hong Kong/258/97.Interpretation Although direct contact between the sick child and affected chickens has not been established, our results suggest transmission of the virus from infected chickens to the child without intermediate mammalian host as a acting vessel". This event illustrates the intensive global influenza surveillance.