Assessment of anti-angiogenic and anti-tumoral potentials of Origanum onites L. essential oil
Assessment of anti-angiogenic and anti-tumoral potentials of Origanum onites L. essential oil
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DOI：
10.1016/j.fct.2012.03.074
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发表时间：
2012-06-01
影响因子：
4.3
通讯作者：
Koparal, Ayse Tansu
中科院分区：
文献类型：
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作者：
Bostancioglu, Rakibe Beklem;Kurkcuoglu, Mine;Koparal, Ayse Tansu
具有抗血管生成和毒性小的药用植物和烹饪草药已经变得重要。无毒的抗血管生成植物化学物质通过防止新血管的形成以支持肿瘤生长而用于对抗癌症。我们研究了牛至挥发油（OOEO），为可能的抗血管生成活性。采用气相色谱法（GC）和气相色谱-质谱法（GC/MS）对OOEO进行分析。以大鼠脂肪组织内皮细胞（RATECs）和5 RP 7（c-H-ras转化的大鼠胚胎成纤维细胞）为实验对象，研究了OOEO的抗增殖活性（MTT法，3-（4，5-二甲基-2-噻唑）-2，5-二苯基-2H-溴化四氮唑）、抗血管生成活性（试管形成法）、细胞迁移抑制能力（迁移法）和凋亡潜能（DAPI染色）。结果表明，OOEO可明显抑制5 RP 7细胞的细胞活力并诱导其凋亡，并可阻断RATEC的体外管状形成和迁移。这些结果意味着具有抗血管生成活性的OOEO可能用于预防血管生成相关疾病和对抗癌症。(C)2012爱思唯尔有限公司保留所有权利。
Medicinal plants and culinary herbs with anti-angiogenic and little toxicity properties have gained importance. Non-toxic anti-angiogenic phytochemicals are useful in combating cancer by preventing the formation of new blood vessels to support the tumor growth. We have investigated the essential oil of Origanum onites L (OOEO), for a possible anti-angiogenic activity. OOEO was analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC/MS). The anti-proliferative activities (by MTT assay, 3-(4,5-dimethy1-2-thiazol)-2,5-dipheny1-2H-tetrazolium bromide), anti-angiogenic activities (by tube formation assay), cell migration inhibiting capability (migration assay) and apoptotic potential (DAPI staining) of OOEO were evaluated on rat adipose tissue endothelial cells (RATECs) and 5RP7 (c-H-ras transformed rat embryonic fibroblasts) cells. Our results revealed that OOEO could markedly inhibit cell viability and induced apoptosis of 5RP7 cells and also could block in vitro tube formation and migration of RATEC. These results imply that OOEO having anti-angiogenic activity might be useful in preventing angiogenesis-related diseases and in combating cancer. (C) 2012 Elsevier Ltd. All rights reserved.