Insulin rapidly increases diacylglycerol by activating de novo phosphatidic acid synthesis.
Insulin rapidly increases diacylglycerol by activating de novo phosphatidic acid synthesis.
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胰岛素通过激活磷脂酸从头合成来快速增加二酰甘油。
DOI：
10.1126/science.3107122
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Cooper,DR
中科院分区：
文献类型：
--
作者：
Farese,RV;Konda,TS;Davis,JS;Standaert,ML;Pollet,RJ;Cooper,DR
研究了胰岛素增加BC3H-1心肌细胞二酰甘油的作用机制。当磷脂的[3 H]花生四烯酸标记作为磷脂酶C激活的指示剂时，在胰岛素治疗开始后的0.5~10分钟内，观察到[3 H]二酰甘油的一过性升高。以[3 H]甘油标记为磷脂合成的指示剂，[3 H]二酰甘油在1分钟时升高最大，并持续升高20分钟。[~3H]甘油标记的甘油二酯主要直接来源于磷脂酸。胰岛素在5到10秒内增加了磷脂酸的从头合成；在1分钟内，这种合成是对照组的60倍。因此，二酰甘油最初的增加是由于磷脂的水解率增加和磷脂酸从头合成的突然增加。5到10分钟后，从头合成磷脂酸继续成为二酰基甘油的主要来源。胰岛素的这两种磷脂效应似乎对产生二酰甘油和其他磷脂衍生的细胞内信号物质很重要。
The mechanisms whereby insulin increases diacylglycerol in BC3H-1 myocytes were examined. When [3H]arachidonate labeling of phospholipids was used as an indicator of phospholipase C activation, transient increases in [3H]diacylglycerol were observed between 0.5 and 10 minutes after the onset of insulin treatment. With [3H]glycerol labeling as an indicator of de novo phospholipid synthesis, [3H]diacylglycerol was increased maximally at 1 minute and remained elevated for 20 minutes. [3H]Glycerol-labeled diacylglycerol was largely derived directly from phosphatidic acid. Insulin increased de novo phosphatidic acid synthesis within 5 to 10 seconds; within 1 minute, this synthesis was 60 times greater than that of controls. Thus, the initial increase in diacylglycerol is due to both increased hydrolysis of phospholipids and a burst of de novo phosphatidic acid synthesis. After 5 to 10 minutes, de novo phosphatidic acid synthesis continues as a major source of diacylglycerol. Both phospholipid effects of insulin seem important for generating diacylglycerol and other phospholipid-derived intracellular signaling substances.