The RcsC sensor kinase is required for normal biofilm formation in Escherichia coli K-12 and controls the expression of a regulon in response to growth on a solid surface
The RcsC sensor kinase is required for normal biofilm formation in Escherichia coli K-12 and controls the expression of a regulon in response to growth on a solid surface
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DOI：
10.1046/j.1365-2958.2003.03815.x
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发表时间：
2003-12-01
影响因子：
3.6
通讯作者：
Clarke, DJ
中科院分区：
文献类型：
--
作者：
Ferrières, L;Clarke, DJ
通常发现细菌与表面相关，作为称为生物膜的固着细菌群落，并且生物膜的形成可以分为不同的阶段，每个阶段都需要特定基因的表达。胞外多糖（EPS）的产生对于生物膜的成熟是重要的，并且由大肠杆菌（和其他革兰氏阴性菌）中的Rcs双组分途径控制。在这项研究中，我们首次表明，RcsC传感器激酶是大肠杆菌正常生物膜发育所必需的。杆菌此外，利用DNA宏阵列技术和转录融合分析的组合，我们表明，在E.杆菌对RcsC调节子的计算机分析预测，50%的基因编码的蛋白质定位于E.大肠杆菌或具有影响细菌表面的结构/性质的活性，例如柯拉酸的产生。此外，我们还表明，RcsC在固体表面上的生长过程中被激活。因此，我们认为RcsC传感器激酶可能在固体表面生长和生物膜形成过程中细菌表面的重塑中发挥重要作用。
Bacteria are often found associated with surfaces as sessile bacterial communities called biofilms, and the formation of a biofilm can be split up into different stages each requiring the expression of specific genes. The production of extracellular polysaccharides (EPS) is important for the maturation of biofilms and is controlled by the Rcs two-component pathway in Escherichia coli (and other Gram-negative bacteria). In this study, we show, for the first time, that the RcsC sensor kinase is required for normal biofilm development in E. coli. Moreover, using a combination of DNA macroarray technology and transcriptional fusion analysis, we show that the expression of > 150 genes is controlled by RcsC in E. coli. In silico analyses of the RcsC regulon predicts that 50% of the genes encode proteins that are either localized to the envelope of E. coli or have activities that affect the structure/properties of the bacterial surface, e.g. the production of colanic acid. Moreover, we also show that RcsC is activated during growth on a solid surface. Therefore, we suggest that the RcsC sensor kinase may play an important role in the remodelling of the bacterial surface during growth on a solid surface and biofilm formation.