Generating and evaluating a ranked candidate gene list for potential vertebrate heart field regulators.
Generating and evaluating a ranked candidate gene list for potential vertebrate heart field regulators.
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DOI：
10.1016/j.gdata.2015.09.015
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发表时间：
2015-12
期刊：
影响因子：
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通讯作者：
MacRae CA
中科院分区：
文献类型：
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作者：
Musso G;Mosimann C;Panáková D;Burger A;Zhou Y;Zon LI;MacRae CA
脊椎动物心脏由两种不同的心肌细胞谱系发育而来，分别来自第一和第二心脏场（FHF和SHF）。FHF形成了原始的心脏管，而从SHF中添加的细胞允许在管的两极延伸。最初被认为是高等脊椎动物的独有特征，最近的研究表明，包括斑马鱼在内的低等脊椎动物也存在明显的FHF和SHF。我们发现，在高等脊椎动物中，调节分隔和腔室形成的关键转录因子，包括Tbx5和Pitx2，影响斑马鱼心脏管中FHF和SHF的相对贡献。为了鉴定心脏场迁移的分子调节因子，我们在胚胎斑马鱼中抑制tbx5a和pitx2ab后使用了基于微阵列的表达谱分析（Mosimann & Panakova, et al, 2015; GSE70750）。在这里，我们更详细地描述了用于处理、优先排序和分析功能富集表达数据的程序。
The vertebrate heart develops from two distinct lineages of cardiomyocytes that arise from the first and second heart fields (FHF and SHF, respectively). The FHF forms the primitive heart tube, while adding cells from the SHF allows elongation at both poles of the tube. Initially seen as an exclusive characteristic of higher vertebrates, recent work has demonstrated the presence of a distinct FHF and SHF in lower vertebrates, including zebrafish. We found that key transcription factors that regulate septation and chamber formation in higher vertebrates, including Tbx5 and Pitx2, influence relative FHF and SHF contributions to the zebrafish heart tube. To identify molecular modulators of heart field migration, we used microarray-based expression profiling following inhibition of tbx5a and pitx2ab in embryonic zebrafish (Mosimann & Panakova, et al, 2015; GSE70750). Here, we describe in more detail the procedure used to process, prioritize, and analyze the expression data for functional enrichment.