TRANSPOSITION OF AN ANTIBIOTIC-RESISTANCE ELEMENT IN MYCOBACTERIA
TRANSPOSITION OF AN ANTIBIOTIC-RESISTANCE ELEMENT IN MYCOBACTERIA
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DOI：
10.1038/345739a0
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发表时间：
1990-06-21
期刊：
影响因子：
64.8
通讯作者：
GICQUEL, B
中科院分区：
文献类型：
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作者：
MARTIN, C;TIMM, J;GICQUEL, B
细菌对抗生素的耐药性通常是质粒介导的，相关的耐药基因由转座因子编码。分枝杆菌包括人类病原体结核分枝杆菌和M.麻风对许多抗生素具有抗性，并且它们的细胞表面结构被认为是大范围抗性表型的主要原因。抗生素耐药质粒迄今尚未涉及分枝杆菌对抗生素的耐药性。然而，在快速生长的物种中检测到了氨基糖苷类乙酰转移酶1和磷酸转移酶1等代谢修饰活性2，3。在大多数快速和缓慢生长的分枝杆菌中也发现了β-内酰胺酶。迄今为止，还没有分枝杆菌的耐药性基因被分离和鉴定。我们现在报告的分离，克隆和测序的遗传区域负责耐磺胺类药物在M。很偶然。该区域还含有一个开放阅读框，与编码位点特异性整合酶的Tn 16964（Tn 21家族成员）中存在的开放阅读框同源5，6。分枝杆菌耐药元件的侧翼是880个碱基对的重复序列，类似于在革兰氏阳性菌和革兰氏阴性菌中发现的IS 6家族的插入元件。插入元件被证明转座到一个相关的快速增长的物种，M染色体的不同位点。恶臭该元件的特性应允许转座子诱变在分枝杆菌毒力和相关问题的分析。
BACTERIAL resistance to antibiotics is often plasmid-mediated and the associated resistance genes encoded by transposable elements. Mycobacteria, including the human pathogensMycobacterium tuberculosisandM. leprae, are resistant to many antibiotics, and their cell-surface structure is believed to be largely responsible for the wide range of resistance phenotypes. Antibiotic-resistance plasmids have so far not been implicated in resistance of mycobacteria to antibiotics. Nevertheless, antibiotic-modifying activities such as aminoglycoside acetyltransferases1and phosphotransferases1have been detected in fast-growing species2,3. β-lactamases have also been found in most fast- and slow-growing mycobacteria. To date no mycobacterial antibiotic-resistance genes have been isolated and characterized. We now report the isolation, cloning and sequencing of a genetic region responsible for resistance to sulphonamides inM. fortuitum. This region also contains an open reading frame homologous to one present in Tn16964(member of the Tn21family) which encodes a site-specific integrase5,6. The mycobacterial resistance element is flanked by repeated sequences of 880 base pairs similar to the insertion elements of the IS6family found in Gram+and Gram-bacteria. The insertion element is shown to transpose to different sites in the chromosome of a related fast-growing species,M. smegmatis. The characterization of this element should permit transposon mutagenesis in the analysis of mycobacterial virulence and related problems.