Urinary expression of long non-coding RNA TUG1 in non-diabetic patients with glomerulonephritides
Urinary expression of long non-coding RNA TUG1 in non-diabetic patients with glomerulonephritides
复制标题
DOI：
10.3892/br.2020.1393
复制
发表时间：
2021-01-01
影响因子：
2.3
通讯作者：
Echavarria, Raquel
中科院分区：
文献类型：
--
作者：
Javier Salazar-Torres, Fernando;Medina-Perez, Miguel;Echavarria, Raquel
代谢改变在肾脏疾病的发病机制中起重要作用。长链非编码RNA（lncRNA）牛磺酸上调基因1（TUG1）是足细胞健康和线粒体生物发生的已知调节因子。尽管TUG1在糖尿病肾病模型中保护足细胞损失，但尿TUG1表达是否与诊断为肾小球肾炎的非糖尿病患者的临床和组织病理学结果相关尚不清楚。在本研究中，TUG 1，足细胞特异性标志物（nephrin和podocin）和线粒体生物发生相关的mRNA（线粒体转录因子A，细胞色素C氧化酶亚基5A和过氧化物酶体增殖物激活受体γ共激活因子1 α）的表达进行了检查，在非糖尿病患者的尿沉渣活检证实肾小球肾炎和健康对照。尿TUG1的表达在肾小球肾炎患者中显著降低，特别是那些诊断为局灶节段性肾小球硬化症（FSGS）的患者。此外，TUG 1水平与足细胞特异性标志物和与线粒体生物发生相关的mRNA的尿表达相关。尿沉渣中TUG 1表达的缺失与FSGS密切相关，突出了这种lncRNA及其线粒体生物发生相关靶点作为评估足细胞病的非侵入性生物标志物的潜力。
Metabolic alterations serve a significant role in the pathogenesis of kidney disease. Long non-coding RNA (lncRNA) taurine upregulated gene 1 (TUG1) is a known regulator of podocyte health and mitochondrial biogenesis. Although TUG1 protects against podocyte loss in models of diabetic nephropathy, it is unknown if urinary TUG1 expression is associated with clinical and histopathological findings in non-diabetic patients diagnosed with glomerulonephritides. In the present study, the expression of TUG1, podocyte-specific markers (nephrin and podocin) and mitochondrial biogenesis-associated mRNAs (transcription factor A mitochondrial, cytochrome C oxidase subunit 5A and peroxisome proliferator-activated receptor gamma coactivator 1 alpha) were examined in urinary sediment of non-diabetic patients with biopsy-confirmed glomerulonephritides and healthy controls. Urinary expression of TUG1 was significantly lower in patients with glomerulonephritides, particularly those diagnosed with Focal Segmental Glomerulosclerosis (FSGS). Furthermore, TUG1 levels were associated with urinary expression of podocyte-specific markers and mRNAs associated with mitochondrial biogenesis. Loss of TUG1 expression in urinary sediment was strongly associated with FSGS, highlighting the potential of this lncRNA and its mitochondrial biogenesis-associated targets as non-invasive biomarkers of assessing podocytopathy.