Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin.
Se-SAD serial femtosecond crystallography datasets from selenobiotinyl-streptavidin.
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DOI：
10.1038/sdata.2017.55
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发表时间：
2017-04-25
期刊：
影响因子：
9.8
通讯作者：
Hunter MS
中科院分区：
文献类型：
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作者：
Yoon CH;DeMirci H;Sierra RG;Dao EH;Ahmadi R;Aksit F;Aquila AL;Batyuk A;Ciftci H;Guillet S;Hayes MJ;Hayes B;Lane TJ;Liang M;Lundström U;Koglin JE;Mgbam P;Rao Y;Rendahl T;Rodriguez E;Zhang L;Wakatsuki S;Boutet S;Holton JM;Hunter MS
我们提供了使用相干 X 射线成像 (CXI) 仪器在直线加速器相干光源 (LCLS) 上记录的硒生物素-链霉亲和素 (Se-B SA) 共晶数据集的详细描述，用于硒单波长异常衍射 (Se-SAD) 结构测定。 Se-B SA 因其生物素和链霉亲和素之间的高亲和力而被选为模型系统，其中生物素分子 (C10H16N2O3S) 中的硫原子被硒取代。使用串行样品室设置以三种不同的传输率（100%、50% 和 10%）收集数据集，该设置允许两个样品室（前室和后室）同时运行。 Se-B SA 的衍射图记录分辨率为 1.9 Å。该数据集可通过相干 X 射线成像数据库 (CXIDB) 以及 LCLS 计算节点公开获取，作为研究和算法开发的资源。
We provide a detailed description of selenobiotinyl-streptavidin (Se-B SA) co-crystal datasets recorded using the Coherent X-ray Imaging (CXI) instrument at the Linac Coherent Light Source (LCLS) for selenium single-wavelength anomalous diffraction (Se-SAD) structure determination. Se-B SA was chosen as the model system for its high affinity between biotin and streptavidin where the sulfur atom in the biotin molecule (C10H16N2O3S) is substituted with selenium. The dataset was collected at three different transmissions (100, 50, and 10%) using a serial sample chamber setup which allows for two sample chambers, a front chamber and a back chamber, to operate simultaneously. Diffraction patterns from Se-B SA were recorded to a resolution of 1.9 Å. The dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) and also on LCLS compute nodes as a resource for research and algorithm development.