THE MOUSE SEGMENTATION GENE KR ENCODES A NOVEL BASIC DOMAIN LEUCINE-ZIPPER TRANSCRIPTION FACTOR
THE MOUSE SEGMENTATION GENE KR ENCODES A NOVEL BASIC DOMAIN LEUCINE-ZIPPER TRANSCRIPTION FACTOR
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DOI：
10.1016/0092-8674(94)90033-7
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发表时间：
1994-12-16
期刊：
影响因子：
64.5
通讯作者：
BARSH, GS
中科院分区：
文献类型：
--
作者：
CORDES, SP;BARSH, GS
小鼠kreisler（kr）突变导致尾部后脑的分段异常和内耳发育缺陷。基于在原始kr等位基因中发现的倒位，我们选择了在发育中的尾侧后脑中高度表达的候选cDNA。该cDNA编码碱性结构域-亮氨酸拉链（bZIP）转录因子，并通过分析化学诱变产生的第二个kr等位基因（其中丝氨酸取代所有已知bZIP家族成员的DNA结合结构域中保守的天冬酰胺残基）证实代表kr基因。kr的身份，表达和突变表型表明在轴向模式的早期作用，并提供了对支配后脑和耳发育的分子和胚胎学机制的见解。
The mouse kreisler(kr) mutation causes segmentation abnormalities in the caudal hindbrain and defective inner ear development. Based on an inversion discovered in the original kr allele, we selected a candidate cDNA highly expressed in the developing caudal hindbrain. This cDNA encodes a basic domain-leucine zipper (bZIP) transcription factor and was confirmed to represent the kr gene by analysis of a second kr allele, generated by chemical mutagenesis, in which a serine is substituted for an asparagine residue conserved in the DNA-binding domain of all known bZIP family members. The identity, expression, and mutant phenotype of kr indicate an early role in axial patterning and provide insights into the molecular and embryologic mechanisms that govern hindbrain and otic development.