Detection of oral bacteria on the tongue dorsum using PCR amplification of 16S ribosomal RNA and its association with systemic disease in middle-aged and elderly patients
Detection of oral bacteria on the tongue dorsum using PCR amplification of 16S ribosomal RNA and its association with systemic disease in middle-aged and elderly patients
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DOI：
10.3892/br.2018.1175
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发表时间：
2019-01-01
影响因子：
2.3
通讯作者：
Sugiyama, Masaru
中科院分区：
文献类型：
--
作者：
Su, Cheng-Yih;Shigeishi, Hideo;Sugiyama, Masaru
口腔健康和全身性疾病之间的联系在文献中得到了认可。本研究旨在阐明中老年人舌背口腔细菌与口腔健康及全身性疾病相关因素之间的关系。本文对广岛大学医院口腔卫生科70例患者（平均年龄69.5岁，45 ~ 92岁）的细菌数量、口腔健康状况与全系统疾病之间的关系进行了初步调查。利用细菌16S核糖体RNA基因，采用实时聚合酶链反应（PCR）技术定量细菌数量。采用PCR检测牙周病相关细菌的DNA。口腔细菌数量与舌面水分水平呈微负相关[Spearman's秩相关系数(R)=-0.131， P=0.28]。有探诊出血（BOP）或探诊深度为>= 4mm （PD）的患者，其牙龈卟啉单胞菌（P. gingivalis）阳性率分别为50.0%和51.1%，高于无BOP或无PD的患者
The association between oral health and systemic disease is recognized in the literature. The present study aimed to clarify the association between oral bacteria on the tongue dorsum and factors associated with oral health and systemic disease in middle-aged and elderly patients. The association between bacterial numbers, oral health status and systemic disease was preliminarily investigated in 70 patients (mean age, 69.5 years; range, 45-92 years) who visited the Department of Oral Health, Hiroshima University Hospital (Hiroshima, Japan). The bacterial 16S ribosomal RNA gene was employed to quantitate bacterial numbers using real-time polymerase chain reaction (PCR). PCR was also performed to detect the DNA of periodontal disease-related bacteria. Oral bacterial numbers were marginally negatively correlated with moisture levels on the tongue surface [Spearman's rank con-elation coefficient (R)=-0.131, P=0.28). Subjects with bleeding on probing (BOP) or a >= 4 mm probing depth (PD) exhibited higher Porphyromonas gingivalis (P. gingivalis)-positive rates (50.0 and 51.1%, respectively) than those without BOP or a