Isolation and Expression Analysis of Anthocyanin Biosynthesis Genes from the Red Chinese Sand Pear, Pyrus pyrifolia Nakai cv. Mantianhong, in Response to Methyl Jasmonate Treatment and UV-B/VIS Conditions
Isolation and Expression Analysis of Anthocyanin Biosynthesis Genes from the Red Chinese Sand Pear, Pyrus pyrifolia Nakai cv. Mantianhong, in Response to Methyl Jasmonate Treatment and UV-B/VIS Conditions
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红沙梨花青素生物合成基因的分离及表达分析
DOI：
10.1007/s11105-013-0652-6
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发表时间：
2014-04-01
影响因子：
2.1
通讯作者：
Teng, Yuanwen
中科院分区：
文献类型：
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作者：
Qian, Minjie;Yu, Bo;Teng, Yuanwen
研究了UV-B/维斯对红砂梨品种“满天红”花色素苷合成相关基因表达的影响，以及植物激素茉莉酸甲酯（MJ）在其中的作用。从该品种中分离到4个花青素合成相关基因的全长cDNA和3个部分cDNA片段。PpPAL 2和PpCHS 2与已报道的PpPAL 1和PpCHS 1序列具有相当大的序列同一性。其他基因显示出低显着的序列同一性，以其他报告的成员相同的基因家族。成熟梨果实套袋后采收，然后用于采后处理。果实经蒸馏水浸泡后，再用UV-B/维斯光照射，或先用MJ处理后再用UV-B/维斯光照射。以浸泡在蒸馏水中并置于黑暗中的果实为对照。在UV-B/维斯照射期间，梨中大多数花色素苷合成基因的表达量与花色素苷的积累量相当，但PpDFR 1、PpDFR 2、PpbHLH和PpWD 40没有表达。MJ处理对花色素苷生物合成相关基因的表达有两种影响。使达峰时间提前，mRNA水平增加。花色素苷生物合成相关基因家族的每个成员都表现出不同的表达谱。还分析了调节MJ介导的植物反应的两个基因PpJAZ 1和PpCOI 1的表达模式。更明显的下调和上调PpJAZ 1和PpCOI 1，分别检测到在2天的照射（DI）的水果进行MJ处理，因为大多数基因的表达与花青素的生物合成在这个时候达到高峰。我们还发现MJ和UV-B/维斯的调控发生在转录水平。这些发现将提高我们对花青素苷生物合成的分子机制的理解，这反过来可能有助于开发新的方法来改善水果颜色。
The effects of UV-B/VIS on the expression of genes involved in anthocyanin biosynthesis in the red Chinese sand pear cultivar 'Mantianhong', and the role played by the plant hormone methyl jasmonate (MJ) in this effect, were investigated. Four full-length cDNAs and three partial cDNA fragments of genes involved in anthocyanin biosynthesis were isolated from the cultivar. PpPAL2 and PpCHS2 showed considerable sequence identity to the reported PpPAL1 and PpCHS1 sequences. Other genes showed low pronounced sequence identity to other reported members of the same gene family. Bagged mature pear fruits were harvested and then used for postharvest treatment. Fruits were immersed in distilled water, then irradiated with UV-B/VIS light; or treated with MJ first, and then irradiated with UV-B/VIS light. Fruits immersed in distilled water and kept in darkness were used as controls. During UV-B/VIS irradiation, most anthocyanin biosynthesis genes were upregulated in pears in quantities commensurate with the accumulation of anthocyanin, but PpDFR1, PpDFR2, PpbHLH, and PpWD40 were not. MJ treatment had two effects on the expression of genes related to anthocyanin biosynthesis. It advanced the peak time and increased mRNA levels. Each family member of genes involved in anthocyanin biosynthesis showed a differential expression profile. The expression patterns of two genes regulating MJ-mediated plant responses, PpJAZ1 and PpCOI1, were also analyzed. More obvious downregulation and upregulation of PpJAZ1 and PpCOI1, respectively, were detected at 2 days of irradiation (DI) in fruits that were subjected to MJ treatment, as the expressions of most genes related to anthocyanin biosynthesis peaked at this time. We also showed that the regulation of MJ and UV-B/VIS occurred at the transcriptional level. These findings should improve our understanding of the molecular mechanism(s) underlying anthocyanin biosynthesis, which may in turn facilitate the development of new methods to improve fruit color.