Biodegradability and cell-mediated contraction of porous collagen scaffolds: The effect of lysine as a novel crosslinking bridge
Biodegradability and cell-mediated contraction of porous collagen scaffolds: The effect of lysine as a novel crosslinking bridge
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DOI：
10.1002/jbm.a.30170
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发表时间：
2004-11-01
影响因子：
4.9
通讯作者：
Shen, JC
中科院分区：
文献类型：
--
作者：
Ma, L;Gao, CY;Shen, JC
采用一种新的交联方法，以赖氨酸为交联桥，分别用水溶性碳二亚胺、1-乙基-3-（3-二甲氨基丙基）碳二亚胺（EDAC）和N-羟基琥珀酰亚胺（NHS）对多孔胶原支架进行交联改性。体外生物降解试验证明，赖氨酸的存在下，EDAC交联支架的生物稳定性大大提高。所得支架的生物稳定性也被阐明为赖氨酸和EDAC/NHS浓度的函数。与Col-DHT相比，Col-EDAC和Col/Lys抗细胞介导收缩（CMC）的能力明显增强。而Col-EDAC与Col/Lys的CMC无明显差异。扫描电镜观察表明，成纤维细胞接种后，交联支架的微观结构基本保持不变。结果，MTT分析证明，与DHT处理的支架相比，Col/Lys支架中的成纤维细胞增殖更快，假设细胞活力保持在相似的水平。组织学切片结果表明，Col/Lys支架具有促进细胞浸润和增殖的能力。所有这些结果表明，这种新的交联方法是获得具有改善的生物稳定性和更稳定的结构的胶原支架的有效方法，其可以抵抗细胞介导的收缩。(C)2004 Wiley Periodicals，Inc.
A novel crosslinking method was adopted to modify the porous collagen scaffolds by using a water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDAC) and N-hydroxysuccinimide (NHS) in the presence of lysine, which functions as a crosslinking bridge. In vitro biodegradation tests proved that in the presence of lysine the biostability of the EDAC crosslinked scaffolds was greatly enhanced. The biostability of the resultant scaffolds was also elucidated as a function of the concentrations of lysine and EDAC/NHS. Compared to the Col-DHT, the ability of the Col-EDAC and the Col/Lys to resist cell-mediated contraction (CMC) was greatly enhanced. Yet no obvious difference between the Col-EDAC and the Col/Lys was found with respect to CMC. SEM observations showed that the microstructure of the crosslinked scaffolds could be largely preserved after fibroblast seeding. As a result, MTT assays proved that the fibroblasts in the Col/Lys scaffolds proliferated faster compared to the DHT-treated one on the assumption that the cell viability was preserved to a similar level. Histological section results indicated that the Col/Lys scaffolds had the ability to accelerate the cell infiltration and proliferation. All these results demonstrated that this novel crosslinking method is an effective way to achieve a collagen scaffold with improved biostability and a more stable structure, which can resist cell-mediated contraction. (C) 2004 Wiley Periodicals, Inc.