Dopaminergic signaling in prefrontal cortex contributes to the antidepressant effect of electroacupuncture: An iTRAQ‐based proteomics analysis in a rat model of CUMS
Dopaminergic signaling in prefrontal cortex contributes to the antidepressant effect of electroacupuncture: An iTRAQ‐based proteomics analysis in a rat model of CUMS
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前额皮质中的多巴胺能信号传导有助于电针的抗抑郁作用：在 CUMS 大鼠模型中基于 iTRAQ™ 的蛋白质组学分析
DOI：
10.1002/ar.24732
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Shanshan Qu
中科院分区：
文献类型：
--
作者：
Jialing Zhang;Jiping Zhang;Zhinan Zhang;Yu Zheng;Zheng Zhong;Zengyu Yao;Xiaowen Cai;Lixing Lao;Yong Huang;Shanshan Qu
电针（EA）被用作抑郁症的辅助治疗。本研究旨在探讨EA在成年雄性Wistar大鼠慢性不可预测轻度应激（CUMS）抑郁模型中的作用及机制。采用等压标记相对定量和绝对定量（iTRAQ）方法对前额叶皮层（PFC）蛋白进行蛋白质组学分析，观察苏木精和伊红染色（H&E）后PFC神经元数量和透射电镜下突触形态学变化，探讨其潜在机制。结果显示，EA + paroxetine （EA + Par）治疗1周可显著缓解抑郁样快感缺乏症状，改善焦虑样行为，并伴有突触形态的改善和PFC神经元的显著增加。此外，EA或帕罗西汀单独干预2周后显着缓解快感缺乏症状。此外，iTRAQ分析显示，EA治疗1周后，CUMS大鼠的多巴胺能信号明显改变。作为该途径的关键酶，EA + Par处理1周后，芳香- 1 -氨基酸脱羧酶（aromatic‐l‐amino‐acid decarboxylase， DDC）显著上调。这些发现提示PFC的多巴胺能信号通路可能参与EA的抗抑郁机制。
Electroacupuncture (EA) is used as an adjunctive treatment for depression. This study was conducted to evaluate the efficacy and mechanisms of EA in the depressive rat model induced by chronic unpredictable mild stress (CUMS) in male adult Wistar rats. The underlying mechanisms were explored by using isobaric tags for relative and absolute quantitation (iTRAQ)‐based proteomic analysis of the proteins in the prefrontal cortex (PFC), and observing the number of the PFC neurons stained with hematoxylin and eosin (H&E) and synaptic morphological changes under transmission electron microscopy (TEM). The results showed that EA plus paroxetine (EA + Par) for 1 week significantly relieved depression‐like anhedonia symptoms and improved anxiety‐like behavior, accompanied by the improvements in synaptic morphology and a significant increase of PFC neurons. Moreover, EA or paroxetine alone significantly alleviated anhedonia symptoms after 2 weeks of intervention. Additionally, iTRAQ analysis showed that dopaminergic signaling was significantly altered in CUMS rats after 1 week of EA treatment. As the critical enzyme of this pathway, aromatic‐l‐amino‐acid decarboxylase (DDC) was significantly upregulated after the treatment with EA + Par for 1 week. These findings suggested that the dopaminergic signaling pathway in PFC may be involved in the antidepressant mechanisms of EA.
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DOI：
--
发表时间：
2016
期刊：
--
影响因子：
--
作者：
S. Woodward;B. Bereznicki;J. Westbury;L. Bereznicki
通讯作者：
S. Woodward;B. Bereznicki;J. Westbury;L. Bereznicki
影响因子：
4.8
作者：
G. Pandey;Yogesh K. Dwivedi;Xinguo Ren;H. Rizavi;R. Roberts;R. Conley;C. Tamminga
通讯作者：
G. Pandey;Yogesh K. Dwivedi;Xinguo Ren;H. Rizavi;R. Roberts;R. Conley;C. Tamminga
DOI：
10.2147/opth.s2317
发表时间：
2008-09
期刊：
Clinical ophthalmology (Auckland, N.Z.)
影响因子：
--
作者：
Galor A;Leder HA;Thorne JE;Dunn JP
通讯作者：
Dunn JP
影响因子：
4.7
作者：
Lammel S;Lim BK;Malenka RC
通讯作者：
Malenka RC
影响因子：
4.7
作者：
M. Ebrahimzadeh;M. Mansari;P. Blier
通讯作者：
M. Ebrahimzadeh;M. Mansari;P. Blier