A modified Girard derivatizing reagent for universal profiling and trace analysis of aldehydes and ketones by electrospray ionization tandem mass spectrometry
A modified Girard derivatizing reagent for universal profiling and trace analysis of aldehydes and ketones by electrospray ionization tandem mass spectrometry
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DOI：
10.1002/rcm.3175
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发表时间：
2007-01-01
影响因子：
2
通讯作者：
Johnson, David W.
中科院分区：
文献类型：
--
作者：
Johnson, David W.
碘化4-肼基-N，N，N-三甲基-4-氧代丁铵（HTMOB）是一种改进的吉拉德衍生试剂，用于提高电喷雾串联质谱分析醛酮的灵敏度。与吉拉德T试剂相比，测定信号强度增加3.3倍（琥珀酰丙酮）~ 7.0倍（17-羟孕酮）。HTMOB是醛和酮的通用分析试剂。中性丢失59 Da扫描检测从丙酮到皮质类固醇的所有醛和酮。所描述的应用包括分析酮症儿童尿液中的酮、酮酸和酮二酸，以及分析血浆缩醛磷脂中的长链醛。版权所有（c）2007约翰威利父子有限公司。
4-Hydrazino-N,N,N-trimethyl-4-oxobutanaminium iodide (HTMOB) is a modified Girard derivatizing reagent synthesized to improve the sensitivity of analysis of aldehydes and ketones with electrospray ionization tandem mass spectrometry. Compared with Girard T reagent the measured signal intensity increase is between 3.3 times (succinylacetone) and 7.0 times (17-hydroxyprogesterone). HTMOB is a universal profiling reagent for aldehydes and ketones. A neutral loss of 59 Da scan detects all aldehydes and ketones from acetone to corticosteroids. Applications described include the profiling of ketones, ketoacids and ketodiacids in the urine of children with ketosis and the profiling of long-chain aldehydes incorporated in plasma plasmalogens. Copyright (c) 2007 John Wiley & Sons, Ltd.