Contraction of neuronal branching volume: an anatomic correlate of Pavlovian conditioning.
Contraction of neuronal branching volume: an anatomic correlate of Pavlovian conditioning.
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神经元分支体积的收缩：巴甫洛夫条件反射的解剖学相关性。
DOI：
10.1073/pnas.87.4.1611
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发表时间：
1990
影响因子：
11.1
通讯作者：
Carlos Collin
中科院分区：
文献类型：
--
作者：
Daniel L. Alkon;H. Ikeno;J. Dworkin;Donna L. McPhie;J. L. Olds;I. Lederhendler;L. Matzel;Bernard G. Schreurs;A. Kuzirian;Carlos Collin
软体动物Hermissenda crassicornis的联想记忆以前与特定K+电流的变化、蛋白质磷酸化以及mRNA和特定蛋白质合成增加相关，现在发现伴随着单个已识别神经元（内侧）结构的宏观改变。类型B光感受器细胞。训练后4 - 5天，用镍离子电渗注射B细胞，然后用红壬酸处理，用电荷耦合器件（CCD）数字化假彩色图像的光学切片在“盲”条件下测量终端arborizations。边界卷封闭的中型B细胞分支从经典条件动物明确减少与天真的动物或那些训练与未配对刺激的体积相比。分支体积大小与内侧型B细胞索马体的输入阻力相关。这种联想学习引起的结构变化可能与发育背景中描述的“突触消除”共享功能。
Associative memory of the mollusc Hermissenda crassicornis, previously correlated with changes of specific K+ currents, protein phosphorylation, and increased synthesis of mRNA and specific proteins, is here shown to be accompanied by macroscopic alteration in the structure of a single identified neuron, the medial type B photoreceptor cell. Four to five days after training, terminal arborizations of B cells iontophoretically injected with Ni2+ ions and then treated with rubeanic acid were measured with charge-coupled device (CCD)-digitized pseudocolor images of optical sections under "blind" conditions. Boundary volumes enclosing medial-type B-cell arborizations from classically conditioned animals were unequivocally reduced compared with volumes for naive animals or those trained with unpaired stimuli. Branch volume magnitude was correlated with input resistance of the medial type B-cell soma. Such associative learning-induced structural changes may share function with "synapse elimination" described in developmental contexts.