Bacterial populations and processes involved in acetate and propionate consumption in anoxic brackish sediment
Bacterial populations and processes involved in acetate and propionate consumption in anoxic brackish sediment
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DOI：
10.1111/j.1574-6941.2001.tb00792.x
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发表时间：
2001-03-01
影响因子：
4.2
通讯作者：
Cappenberg, TE
中科院分区：
文献类型：
--
作者：
Boschker, HTS;de Graaf, W;Cappenberg, TE
在德国波罗的海Grosser Jasmunder Bodden的缺氧微咸沉积物中研究了乙酸盐和丙酸盐消耗的细菌种群和途径。利用稳定的碳同位素标记的化合物研究了乙酸盐和丙酸盐从孔隙水中的摄取。标记的乙酸盐在丙酸盐摄取实验期间不作为中间体产生，并且丙酸盐消耗不受乙酸盐添加的影响。同时，研究了标记的乙酸盐和丙酸盐掺入磷脂衍生的脂肪酸（PLFA）中，以指示参与这些底物消耗的细菌种群。C-13-乙酸酯标记物主要在偶数PLFA（16：1 omega 7 c、16：0和18：1 omega 7 c）中回收。相反，在与C-13-丙酸盐孵育后，主要标记奇数PLFA（α 15：0、15：0、17：1 ω 6和17：0）和偶数PLFA i16：0。尽管在硫酸盐还原剂中通常发现用丙酸盐标记的单一PLFA，但完整的PLFA标记模式与任何已知菌株都不相似。然而，乙酸盐标记模式与Desuflotomaculum acetoxidans和Desulfofrigus spp.相似，两个消耗醋酸盐的硫酸盐还原剂总之，我们的数据表明，乙酸盐和丙酸盐主要由不同的，专门的硫酸盐还原菌群消耗。(C)2001年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
Bacterial populations and pathways involved in acetate and propionate consumption were studied in anoxic brackish sediment from the Grosser Jasmunder Bodden, German Baltic Sea. Uptake of acetate and propionate from the porewater was studied using stable carbon isotope-labeled compounds. Labeled acetate was not produced as an intermediate during propionate uptake experiments, and propionate consumption was not affected by the addition of acetate. In parallel, incorporation of labeled acetate and propionate into phospholipid-derived Fatty acids (PLFA) was studied to indicate bacterial populations involved in the consumption of these substrates. The C-13-acetate label was mainly recovered in even-numbered PLFA (16:1 omega 7c, 16:0 and 18:1 omega 7c). In contrast, primarily odd-numbered PLFA (a15:0, 15:0, 17:1 omega6 and 17:0) and the even-numbered i16:0 were labeled after incubation with C-13-propionate. Although single PLFA labeled with propionate are commonly found in sulfate reducers, the complete PLFA-labeling pattern does not resemble any of the know strains. However, the acetate-labeling pattern is similar to Desuflotomaculum acetoxidans and Desulfofrigus spp., two acetate-consuming, sulfate reducers. In conclusion, our data suggest that acetate and propionate were predominantly consumed by different, specialized groups of sulfate-reducing bacteria. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.