Adeno-associated virus type 4 (AAV4) targets ependyma and astrocytes in the subventricular zone and RMS
Adeno-associated virus type 4 (AAV4) targets ependyma and astrocytes in the subventricular zone and RMS
复制标题
DOI：
10.1038/sj.gt.3302554
复制
发表时间：
2005-10-01
期刊：
影响因子：
5.1
通讯作者：
Davidson, BL
中科院分区：
文献类型：
--
作者：
Liu, G;Martins, IH;Davidson, BL
脑室下区(SVZ)是成年哺乳动物脑内的神经源性小生境之一。SVZ对神经发生和干细胞治疗的研究很有兴趣。在此，我们报道了重组腺相关病毒4型(AAV4)病毒载体对室管膜和/或星形胶质细胞的特异性转导。将编码β-半乳糖苷酶或绿色荧光蛋白的AAV4载体注入新生和成年C57BL/6小鼠的侧脑室。此外，在成年小鼠身上进行了SVZ注射。AAV4载体显示了室管膜的一种独立于传递途径的特征转导。然而，AAV4病毒注射到SVZ可以靶向GFAP阳性的星形胶质细胞，形成SVZ和吻端迁移流(RMS)中的神经胶质管。我们的结果介绍了AAV4作为一种新的工具来操纵RMS中的神经胶质细胞。
The subventricular zone (SVZ) is one of the neurogenic niches in the adult mammalian brain. The SVZ is of interest for studies on neurogenesis and stem cell therapy. Here, we report specific transduction of ependyma and/or astrocytes by recombinant adeno-associated virus type 4 (AAV4) viral vectors. AAV4 vectors encoding beta-galactosidase or eGFP were injected into the lateral ventricles of neonatal and adult C57BL/6 mouse brains. In addition, SVZ injections were conducted on adult mice. AAV4 vectors show a characteristic transduction of the ependyma independent of delivery route. However, AAV4 virus injected into the SVZ targeted GFAP positive astrocytes forming the glial tube in the SVZ and rostral migratory stream (RMS). Our results introduce AAV4 as a new tool by which to manipulate glial cells in the RMS.