Ran alters nuclear pore complex conformation
Ran alters nuclear pore complex conformation
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DOI：
10.1006/jmbi.2000.3891
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发表时间：
2000-07-14
影响因子：
5.6
通讯作者：
Clarke, PR
中科院分区：
文献类型：
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作者：
Goldberg, MW;Rutherford, SA;Clarke, PR
跨核膜的转运通过核孔复合物（NPC）发生，并由可溶性转运因子介导，包括Ran，一种通常在输入期间与GDP结合并在输出时与GTP结合的小GTP酶。NPC结构的动态性质表明它在驱动易位中可能起积极作用。在这里，我们表明，RanGTP，但不是RanGDP的原因NPC结构的改变时，注入非洲爪蟾卵母细胞的细胞质，包括压实的NPC和延长的细胞质丝。RanGTP引起核质蛋白金沿着延伸的细胞质丝的长度的积累，而RanGDP引起周围的NPC的细胞质边缘的积累。这表明一个可能的作用，RAN在改变运输过程中的细胞质丝的构象。(C)北京大学出版社.
Transport across the nuclear membranes occurs through the nuclear pore complex (NPC), and is mediated by soluble transport factors including Ran, a small GTPase that is generally GDP-bound during import and GTP-bound for export. The dynamic nature of the NPC structure suggests a possible active role for it in driving translocation. Here we show that RanGTP but not RanGDP causes alterations of NPC structure when injected into the cytoplasm of Xenopus oocytes, including compaction of the NPC and extension of the cytoplasmic filaments. RanGTP caused accumulation of nucleoplasmin-gold along the length of extended cytoplasmic filaments, whereas RanGDP caused accumulation around the cytoplasmic rim of the NPC. This suggests a possible role for Ran in altering the conformation of the cytoplasmic filaments during transport. (C) 2000 Academic Press.