The maternal effect mutation sesame affects the formation of the male pronucleus in Drosophila melanogaster
The maternal effect mutation sesame affects the formation of the male pronucleus in Drosophila melanogaster
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DOI：
10.1006/dbio.2000.9718
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发表时间：
2000-06-15
影响因子：
2.7
通讯作者：
Couble, P
中科院分区：
文献类型：
--
作者：
Loppin, B;Docquier, M;Couble, P
进入卵母细胞后，在二倍体合子形成之前，精核转化为雄性原核，这是一个涉及有性生殖动物中一系列保守步骤的过程。值得注意的是，雄性配子的一个重大修改。在于高度致密的精子染色质的解凝聚。我们在这里提出的芝麻（ssm），母体效应突变的表型，影响果蝇雄性原核的形成。纯合子ssm（185b）雌性产生的单倍体胚胎只发育有来自母体的染色体。这些单倍体胚胎在胚胎发生结束时死亡。ssm（185b）突变体雌虫产下的卵受精的细胞学分析表明，原核迁移和原核附着均正常发生。然而，雄性原核的显着变化，其染色质未能完全去致密系统地观察到，因此，受影响的雄性原核蜜蜂没有进入第一个有丝分裂纺锤体，这是组织周围的只有母系衍生的染色体。对纤层抗原的免疫检测表明，雄性原核被膜能够在部分去致密的父体染色质周围形成，这表明母体提供的芝麻（+）功能是精子染色质重塑后期所必需的。(C)北京大学出版社.
After entering the oocyte and before the formation of the diploid zygote, the sperm nucleus is transformed into a male pronucleus, a process that involves a series of conserved steps in sexually reproducing animals. Notably, a major modification of the male gamete. lies in the decondensation of the highly compact sperm chromatin. We present here the phenotype of sesame (ssm), a maternal effect mutation which affects the formation of the male pronucleus in Drosophila melanogaster. Homozygous ssm(185b) females produce haploid embryos which develop with only the maternally derived chromosomes. These haploid embryos die at the end of embryogenesis. Cytological analyses of the fertilization in eggs laid by ssm(185b) mutant females showed that both pronuclear migration and pronuclear apposition occurred normally. However, a dramatic alteration of the male pronucleus by which its chromatin failed to fully decondense was systematically observed, Consequently, the affected male pronucleus bees not enter the first mitotic spindle, which is organized around only the maternally derived chromosomes. Immunodetection of lamina antigens indicates that a male pronuclear envelope is able to form around the partially decondensed paternal chromatin, This suggests that the maternally provided sesame(+) function is required for a late stage of sperm chromatin remodeling. (C) 2000 Academic Press.