Development of aliphatic biodegradable photoluminescent polymers
Development of aliphatic biodegradable photoluminescent polymers
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DOI：
10.1073/pnas.0900004106
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发表时间：
2009-06-23
影响因子：
11.1
通讯作者：
Tang, Liping
中科院分区：
文献类型：
--
作者：
Yang, Jian;Zhang, Yi;Tang, Liping
目前的生物可降解聚合物都不能同时用作植入材料和荧光成像探针。本研究的目的是开发脂肪族生物可降解的光致发光聚合物（BPLPs）及其相关的交联变体（CBPLPs）的生物医学应用。BPLP是由生物相容性单体（包括柠檬酸、脂肪族二醇和各种氨基酸）通过方便且具有成本效益的缩聚反应合成的可降解低聚物。BPLP可以进一步交联成弹性体交联聚合物CBPLP。BPLP-Cys和BPLP-Ser与传统的荧光有机染料和量子点相比，具有良好的体外细胞相容性、体内慢性炎症反应小、可控的降解性和高量子产率（高达62.33%）、可调的荧光发射（高达725 nm）和光稳定性。CBPLP-Cys膜的拉伸强度范围为3.25 +/-0.13MPa至6.5 +/-0.8MPa，初始模量范围为3.34 +/-0.15MPa至7.02 +/-1.40MPa。弹性CBPLP-Cys可伸长至240 +/-36%。BPLP-Cys（0.6）（1：1：0.6 OD：CA：Cys）多孔支架的压缩模量为39.60 +/-5.90 KPa，证实了支架的柔软性质。BPLPs还具有用于微/纳米纤维的良好加工性。我们证明了使用BPLP-Ser纳米颗粒（“生物可降解量子点”）进行体外细胞标记和组织工程支架的非侵入性体内成像的可行性。BPLPs和CBPLPs的发展代表了荧光生物材料发展的新方向，并可能影响组织工程、药物递送、生物成像。
None of the current biodegradable polymers can function as both implant materials and fluorescent imaging probes. The objective of this study was to develop aliphatic biodegradable photoluminescent polymers (BPLPs) and their associated cross-linked variants (CBPLPs) for biomedical applications. BPLPs are degradable oligomers synthesized from biocompatible monomers including citric acid, aliphatic diols, and various amino acids via a convenient and cost-effective polycondensation reaction. BPLPs can be further cross-linked into elastomeric cross-linked polymers, CBPLPs. We have shown representatively that BPLP-cysteine (BPLP-Cys) and BPLP-serine (BPLP-Ser) offer advantages over the traditional fluorescent organic dyes and quantum dots because of their preliminarily demonstrated cytocompatibility in vitro, minimal chronic inflammatory responses in vivo, controlled degradability and high quantum yields ( up to 62.33%), tunable fluorescence emission ( up to 725 nm), and photostability. The tensile strength of CBPLP-Cys film ranged from 3.25 +/- 0.13 MPa to 6.5 +/- 0.8 MPa and the initial Modulus was in a range of 3.34 +/- 0.15 MPa to 7.02 +/- 1.40 MPa. Elastic CBPLP-Cys could be elongated up to 240 +/- 36%. The compressive modulus of BPLP-Cys (0.6) (1:1:0.6 OD:CA:Cys) porous scaffold was 39.60 +/- 5.90 KPa confirming the soft nature of the scaffolds. BPLPs also possess great processability for micro/nanofabrication. We demonstrate the feasibility of using BPLP-Ser nanoparticles ("biodegradable quantum dots'') for in vitro cellular labeling and noninvasive in vivo imaging of tissue engineering scaffolds. The development of BPLPs and CBPLPs represents a new direction in developing fluorescent biomaterials and could impact tissue engineering, drug delivery, bioimaging.