Divalent cations increase the conjugation efficiency of the incompatibility P-7 group plasmid pCAR1 among different Pseudomonas hosts
Divalent cations increase the conjugation efficiency of the incompatibility P-7 group plasmid pCAR1 among different Pseudomonas hosts
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二价阳离子提高了不相容性P-7组质粒pCAR1在不同假单胞菌宿主之间的缀合效率
DOI：
10.1099/mic.0.000583
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发表时间：
2018
期刊：
影响因子：
1.5
通讯作者：
Nojiri H
中科院分区：
文献类型：
--
作者：
Sakuda A;Suzuki-Minakuchi C;Matsui K;Takahashi Y;Okada K;Yamane H;Shintani M;Nojiri H
不相容（Inc） P-7基团质粒pCAR1在Ca2+和Mg2+二价离子存在的人工微环境中可以有效地在细菌之间转移。假单胞菌与不同质粒的单配实验表明，二价阳离子对其他质粒（包括pB10和NAH7）的结合效率有促进作用；然而，这种效应在IncP-7质粒中更大。对pCAR1偶联的影响因供体-受体对而异，并且在供体-受体之间明显检测到偶联效率的提高。resinovoransCA10dm4 andP。荧光spf0 -1和受体sp。putidaKT2440和CA10dm4。转录组分析显示，pCAR1基因的表达不响应阳离子的变化，包括参与其转移的thetra/trhgenes。然而，在阳离子限制条件下，供体和受体中编码外膜蛋白的oprhgenes的转录通常会上调。发现pCAR1在KT2440oprHmutant中的偶联频率对阳离子无反应。这种作用通过与OprH基因的互补部分恢复，表明OprH参与了二价阳离子增加pCAR1结合效率的过程。
The incompatibility (Inc) P-7 group plasmid pCAR1 can be efficiently transferred among bacteria in artificial microcosms in the presence of divalent cations Ca2+and Mg2+. One-on-one mating assays betweenPseudomonasstrains with different plasmids showed that the promotion of conjugation efficiency by divalent cations was exhibited in other plasmids, including pB10 and NAH7; however, this effect was larger in IncP-7 plasmids. The impact on pCAR1 conjugation differed according to donor–recipient pairs, and conjugation efficiency promotion was clearly detected between the donorsP. resinovoransCA10dm4 andP.fluorescensPf0-1 and the recipientsP. putidaKT2440 and CA10dm4. Transcriptome analyses showed that pCAR1 gene expression did not respond to cation changes, including thetra/trhgenes involved in its transfer. However, the transcription ofoprHgenes, encoding putative outer-membrane proteins in both the donor and the recipient, were commonly upregulated under cation-limited conditions. The conjugation frequency of pCAR1 in the KT2440oprHmutant was found not to respond to cations. This effect was partially recovered by complementation with theoprHgene, suggesting that OprH is involved in the increase of pCAR1 conjugation efficiency by divalent cations.