INSULIN-RECEPTOR SYNTHESIS AND TURNOVER IN DIFFERENTIATING 3T3-L1 PREADIPOCYTES
INSULIN-RECEPTOR SYNTHESIS AND TURNOVER IN DIFFERENTIATING 3T3-L1 PREADIPOCYTES
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DOI：
10.1073/pnas.77.1.285
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
LANE, MD
中科院分区：
文献类型：
--
作者：
REED, BC;LANE, MD
描述了一种用于分析用重氨基酸（2H，13C和15N）标记的培养细胞中胰岛素受体合成和周转的密度转移方法。通过在CSCL梯度上的等异型谱带分离溶解的新合成的重和旧光受体，然后进行定量。通过该技术在3T3-LI前脂肪细胞中研究了胰岛素受体的合成和周转，在分化过程中会增加胰岛素结合能力。胰岛素结合能力的增加显然是新受体合成的结果。胰岛素受体的半衰期相对较短（6.7 h），并且受体合成速率增加有助于分化过程中胰岛素受体水平的升高。
A density-shift method is described for analyzing insulin receptor synthesis and turnover in cultured cells labeled with heavy amino acids (2H, 13C and 15N). Solubilized newly synthesized heavy and old light receptors were separated by isopycnic banding on CsCl gradients and then quantitated. Insulin receptor synthesis and turnover were studied by this technique in 3T3-Li preadipocytes which undergo an increase in insulin binding capacity during differentiation. The increase in insulin binding capacity is evidently a consequence of new receptor synthesis; the insulin receptor has a relatively short half-life (6.7 h), and an increased rate of receptor synthesis contributes to the increase of insulin receptor level during differentiation.