KIN241:: a gene involved in cell morphogenesis in Paramecium tetraurelia reveals a novel protein family of cyclophilin-RNA interacting proteins (CRIPs) conserved from fission yeast to man
KIN241:: a gene involved in cell morphogenesis in Paramecium tetraurelia reveals a novel protein family of cyclophilin-RNA interacting proteins (CRIPs) conserved from fission yeast to man
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DOI：
10.1046/j.1365-2958.2001.02634.x
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发表时间：
2001-10-01
影响因子：
3.6
通讯作者：
Klotz, C
中科院分区：
文献类型：
--
作者：
Krzywicka, A;Beisson, J;Klotz, C
在本研究中，我们通过功能互补克隆了参与草履虫细胞形态发生、皮质组织和核重组的KIN241基因。预计该基因编码一种新型蛋白，包括亲环蛋白型、肽基脯氨酸异构酶结构域、RNA识别基序、富含谷氨酸和赖氨酸的区域（EK结构域）和c端丝氨酸链。由于该蛋白的同源物存在于pombe Schizosaccharomyces， Caenorhabditis elegans, Drosophila melanogaster， Arabidopsis thaliana和Homo sapiens的基因组中，因此Kin241p预测序列定义了一个新的蛋白家族，我们建议将其称为“CRIP”，即亲环蛋白- rna相互作用蛋白。我们证明，在草履虫中，Kin241p定位于细胞核中，一些核定位信号（NLSs）的缺失减少了该蛋白进入细胞核的运输。突变细胞中不存在Kin241-1蛋白，这表明c端富含丝氨酸的区域负责蛋白质的稳定性。
In this study, we report cloning, by functional complementation of the KIN241 gene involved in Paramecium cell morphogenesis, cortical organization and nuclear reorganization. This gene is predicted to encode a protein of a novel type, comprising a cyclophilin-type, peptidyl-prolyl isomerase domain, an RNA recognition motif, followed by a region rich in glutamate and lysine (EK domain) and a C-terminal string of serines. As homologues of this protein are present in the genomes of Schizosaccharomyces pombe, Caenorhabditis elegans, Drosophila melanogaster, Arabidopsis thaliana and Homo sapiens, the Kin241p predicted sequence defines a new family of proteins that we propose to call 'CRIP', for cyclophilin-RNA interacting protein. We demonstrate that, in Paramecium, Kin241p is localized in the nucleus and that deletion of some nuclear localization signals (NLSs) decreases transport of the protein into the nucleus. No Kin241-1 protein is present in mutant cells, suggesting that the C-terminal serine-rich region is responsible for protein stability.