Isoflurane pretreatment supports hemodynamics and leukocyte rolling velocities in rat mesentery during lipopolysaccharide-induced inflammation
Isoflurane pretreatment supports hemodynamics and leukocyte rolling velocities in rat mesentery during lipopolysaccharide-induced inflammation
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DOI：
10.1213/01.ane.0000104584.91385.1d
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发表时间：
2004-04-01
影响因子：
5.7
通讯作者：
Rich, GF
中科院分区：
文献类型：
--
作者：
Hayes, JK;Havaleshko, DM;Rich, GF
我们推测，异氟烷（ISO）预处理对血管系统的保护作用可能部分归因于白细胞-内皮细胞相互作用的改变。大鼠用戊巴比妥麻醉，然后随机分为四组：对照组、ISO对照组（用1.4%ISO预处理30分钟）、脂多糖（LPS; 10 mg/kg IV）和ISO-LPS（ISO预处理，然后LPS）。制备肠系膜用于活体视频显微镜检查。每小时（基线和0-4 h）测量平均动脉血压（MAP），沿着微循环变量，包括毛细血管后小静脉和小动脉血流速度和白细胞动力学（滚动和粘附白细胞数量以及单个滚动白细胞速度）。ISO预处理可明显减轻LPS后2、4 h的MAP下降（P < 0.05），并增加LPS后2- 4 h的白细胞滚动速度（P < 0.05）。LPS后4小时，ISO-LPS大鼠的白细胞滚动速度比LPS大鼠快200%（63.7 +/- 27.6 mum/s对19.8 +/- 6.4 mum/s）。在对照组大鼠中，ISO预处理对MAP或白细胞滚动速度没有影响，但增加了滚动白细胞的数量。ISO预处理对LPS大鼠的小动脉和毛细血管后微静脉血流速度或LPS或对照大鼠的白细胞粘附没有影响。总之，ISO预处理支持血流动力学和增加白细胞滚动速度，但不改变滚动或粘附白细胞在肠系膜微循环在LPS诱导的炎症。
We hypothesized that the protective effects of isoflurane (ISO) pretreatment on the vasculature may be attributed, in part, to altered leukocyte-endothelial interactions. Rats were anesthetized with pentobarbital and then randomized into four groups: control, ISO-control (pretreatment with 30 min of 1.4% ISO), lipopolysaccharide (LPS; 10 mg/kg IV), and ISO-LPS (ISO pretreatment and then LPS). The mesentery was prepared for intravital videomicroscopy. Mean arterial blood pressure (MAP), along with microcirculatory variables that included postcapillary venular and arteriolar blood flow velocity and leukocyte dynamics (number of rolling and adherent leukocytes and individual rolling leukocyte velocities), were measured hourly (baseline and at 0-4 h). In LPS rats, ISO pretreatment significantly (P < 0.05) attenuated the decrease in MAP at 2 and 4 h after LPS and increased leukocyte rolling velocities after 2-4 h. Four hours after LPS, leukocyte rolling velocities were >200% more rapid (63.7 +/- 27.6 mum/s versus 19.8 +/- 6.4 mum/s) in ISO-LPS versus LPS rats. In control rats, ISO pretreatment had no effect on MAP or leukocyte rolling velocities but increased the number of rolling leukocytes. ISO pretreatment had no effect on arteriolar and postcapillary venular blood flow velocity in LPS rats or leukocyte adherence in LPS or control rats. In conclusion, ISO pretreatment supported hemodynamics and increased leukocyte rolling velocities but did not alter the number of rolling or adherent leukocytes in the mesenteric microcirculation during LPS-induced inflammation.