External forces control mitotic spindle positioning
External forces control mitotic spindle positioning
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DOI：
10.1038/ncb2269
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发表时间：
2011-07-01
影响因子：
21.3
通讯作者：
Piel, Matthieu
中科院分区：
文献类型：
--
作者：
Fink, Jenny;Carpi, Nicolas;Piel, Matthieu
细胞对力的反应对于组织形态发生和稳态是必不可少的。这种反应已在间期细胞中进行了广泛的研究，但仍不清楚力如何影响分裂细胞。我们在人类分裂细胞上使用了显微操作工具的组合，以解决微环境的物理参数在控制细胞分裂轴（组织形态发生的关键要素）中的作用。我们发现，在有丝分裂过程中，施加在细胞体上的力可以指导纺锤体的定向。我们进一步表明，外部约束诱导的动态皮层下肌动蛋白结构，与纺锤体运动的极化。我们认为，细胞分裂根据其机械微环境提供的线索，对齐子细胞与外部力场。
The response of cells to forces is essential for tissue morphogenesis and homeostasis. This response has been extensively investigated in interphase cells, but it remains unclear how forces affect dividing cells. We used a combination of micro-manipulation tools on human dividing cells to address the role of physical parameters of the micro-environment in controlling the cell division axis, a key element of tissue morphogenesis. We found that forces applied on the cell body direct spindle orientation during mitosis. We further show that external constraints induce a polarization of dynamic sub cortical actin structures that correlate with spindle movements. We propose that cells divide according to cues provided by their mechanical micro-environment, aligning daughter cells with the external force field.