Effects of Interleukin-6, Leukemia Inhibitory Factor, and Ciliary Neurotrophic Factor on the Proliferation and Differentiation of Adult Human Myoblasts
Effects of Interleukin-6, Leukemia Inhibitory Factor, and Ciliary Neurotrophic Factor on the Proliferation and Differentiation of Adult Human Myoblasts
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DOI：
10.1007/s10571-007-9247-9
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发表时间：
2008-02
影响因子：
4
通讯作者：
Xuan Wang;Haitao Wu;Zhenxing Zhang;Shu-hong Liu;Jian Yang;Xiao-ping Chen;M. Fan;Xiaomin Wang
中科院分区：
文献类型：
--
作者：
Xuan Wang;Haitao Wu;Zhenxing Zhang;Shu-hong Liu;Jian Yang;Xiao-ping Chen;M. Fan;Xiaomin Wang
我们前期的研究表明，睫状神经营养因子（ciliary neurotrophic factor，CGF）是白细胞介素6（interleukin-6，IL-6）类细胞因子超家族成员，在一定浓度下可抑制成肌细胞向成熟肌管分化。本研究以人成肌细胞为研究对象，探讨了白细胞介素-6（IL-6）、白血病抑制因子（LEIF）和睫状神经营养因子（CNTF）对成肌细胞增殖和分化的影响，以证实这两种细胞因子可能是一种新型的成肌细胞调控因子。结果表明，白细胞介素6、白血病抑制因子和睫状神经营养因子对成人成肌细胞增殖和分化的影响是不同的。10 ng/ml的白血病抑制因子可促进细胞增殖。白血病抑制因子（10或50 ng/ml）和睫状神经营养因子（10或50 ng/ml）可抑制成肌细胞分化。其抑制机制可能是白血病抑制因子和睫状神经营养因子抑制了转录因子MyoD/myf 5的表达，而MyoD/myf 5可调节成肌细胞的分化。本研究为人成肌细胞的基础和临床研究提供了实验和理论依据。
Our previous studies have demonstrated that ciliary neurotrophic factor, a member of the interleukin-6-type cytokine superfamily, could inhibit the differentiation of myoblasts into mature myotubes at a certain concentration. In this study, another two members, interleukin-6 and leukemia inhibitory factor, together with ciliary neurotrophic factor were tested their roles in the proliferation and differentiation of myoblasts derived from the adult human skeletal muscles, in order to confirm that these cytokines might be a new type of regulatory factors on the myoblasts. The results showed that the effects of interleukin-6, leukemia inhibitory factor, and ciliary neurotrophic factor on the proliferation and differentiation of adult human myoblasts were different. Leukemia inhibitory factor in the dose of 10 ng/ml could accelerate the cell proliferation. Leukemia inhibitory factor in the dose of 10 or 50 ng/ml and ciliary neurotrophic factor in the dose of 10 or 50 ng/ml could inhibit the myoblast differentiation. The inhibition mechanism might be that leukemia inhibitory factor and ciliary neurotrophic factor inhibited the expressions of transcription factor MyoD/myf5, which could regulate the myoblast differentiation. This study will provide the experimental and theoretic foundations for the basic and clinical researches about human myoblasts.