Enhanced resection of orthotopic red-fluorescent-protein-expressing human glioma by fluorescence-guided surgery in nude mice.
Enhanced resection of orthotopic red-fluorescent-protein-expressing human glioma by fluorescence-guided surgery in nude mice.
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发表时间：
2013
影响因子：
2
通讯作者：
M. Momiyama;Yukihiko Hiroshima;A. Suetsugu;Y. Tome;Sumiyuki Mii;S. Yano;M. Bouvet;T. Chishima;I. Endo;R. Hoffman
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作者：
M. Momiyama;Yukihiko Hiroshima;A. Suetsugu;Y. Tome;Sumiyuki Mii;S. Yano;M. Bouvet;T. Chishima;I. Endo;R. Hoffman
恶性胶质瘤是最常见的原发性中枢神经系统肿瘤。由于胶质瘤的侵袭性，很难完全切除。在本研究中，我们比较了荧光引导和标准的强光切除原位植入裸鼠的人脑胶质瘤。将表达红色荧光蛋白（RFP）的U87人胶质瘤细胞通过开颅术开窗立体定向注射到裸鼠脑中。癌细胞植入两周后，在荧光引导下或在强光下切除胶质瘤。U87-RFP肿瘤用长工作距离荧光显微镜清晰可见。几乎所有的癌细胞都是使用荧光引导导航去除的，而不会损伤脑组织。相比之下，脑肿瘤在强光下难以可视化，并且在强光手术后许多残留的癌细胞仍留在大脑中。与接受强光手术的小鼠相比，荧光引导手术显著延长了小鼠的存活时间。这些结果表明，荧光引导手术具有治疗脑癌的巨大潜力。
Malignant glioma is the most common type of primary central nervous system cancer. Gliomas are very difficult to completely resect due to their invasiveness. In the present study, we compared fluorescence-guided and standard bright-light resection of a human glioma orthotopically implanted in nude mice. U87 human glioma cells, expressing red fluorescent protein (RFP), were injected stereotactically into the nude mouse brain through a craniotomy open window. Two weeks after cancer-cell implantation, gliomas were resected under fluorescence guidance or under bright light. U87-RFP tumors were clearly visualized with a long-working distance fluorescence microscope. Almost all cancer cells were removed using fluorescence-guided navigation without damage to the brain tissue. In contrast, brain tumors were difficult to visualize under bright light and many residual cancer cells remained in the brain after bright-light surgery. Fluorescence-guided surgery significantly extended the survival of the mice compared to those who underwent bright-light surgery. These results suggest that fluorescence-guided surgery has significant potential for brain cancer treatment.