Characterization of the microbiome of nipple aspirate fluid of breast cancer survivors.
Characterization of the microbiome of nipple aspirate fluid of breast cancer survivors.
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DOI：
10.1038/srep28061
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发表时间：
2016-06-21
影响因子：
4.6
通讯作者：
Lee DJ
中科院分区：
文献类型：
--
作者：
Chan AA;Bashir M;Rivas MN;Duvall K;Sieling PA;Pieber TR;Vaishampayan PA;Love SM;Lee DJ
微生物组影响人类健康和疾病。直到最近，人类的乳腺组织和牛奶被认为是无菌的。在这里，我们研究了乳头抽吸液（NAF）中的微生物及其与乳腺癌的潜在关联。我们使用16S rRNA基因扩增子测序比较了患有乳腺癌病史（BC）（BC）（BC）（BC）（HC）的NAF微生物组。来自BC和HC的NAF微生物组在社区组成方面显示出显着差异。两个操作分类单元（OTU）显示从BC和HC收集的NAF之间的相对丰度差异。在从BC收集的NAF中，Alistipes属的发生率相对较高。相比之下，在HC的NAF中，来自鞘氨加州科家族的未分类属相对丰富。这些发现反映了导管源DNA，因为从卑诗省和HC收集的乳晕样品之间没有差异。此外，与BC相关的微生物具有酶促活性，β-葡萄糖醛酸酶，这可能促进乳腺癌。这是人类乳腺导管液中细菌DNA的第一个报道，也是HC和BC的NAF之间的差异。必须进一步研究导管微生物组及其在乳腺癌中的潜在作用。
The microbiome impacts human health and disease. Until recently, human breast tissue and milk were presumed to be sterile. Here, we investigated the presence of microbes in the nipple aspirate fluid (NAF) and their potential association with breast cancer. We compared the NAF microbiome between women with a history of breast cancer (BC) and healthy control women (HC) using 16S rRNA gene amplicon sequencing. The NAF microbiome from BC and HC showed significant differences in community composition. Two Operational Taxonomic Units (OTUs) showed differences in relative abundances between NAF collected from BC and HC. In NAF collected from BC, there was relatively higher incidence of the genus Alistipes. By contrast, an unclassified genus from the Sphingomonadaceae family was relatively more abundant in NAF from HC. These findings reflect the ductal source DNA since there were no differences between areolar skin samples collected from BC and HC. Furthermore, the microbes associated with BC share an enzymatic activity, Beta-Glucuronidase, which may promote breast cancer. This is the first report of bacterial DNA in human breast ductal fluid and the differences between NAF from HC and BC. Further investigation of the ductal microbiome and its potential role in breast cancer are warranted.