Effects of TGF beta-1 on mouse embryo implantation and expression of H2-D1 and H2-DM.
Effects of TGF beta-1 on mouse embryo implantation and expression of H2-D1 and H2-DM.
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DOI：
10.2741/e95
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Yan-shuai Zhang;Gao-Feng Xiong;Jing-Pian Peng
中科院分区：
文献类型：
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作者：
Yan-shuai Zhang;Gao-Feng Xiong;Jing-Pian Peng
本研究采用小鼠子宫角注射模型，探讨了TGF-β 1对母胎界面免疫环境的影响及对胚胎着床的影响机制。采用实时荧光定量PCR、免疫印迹和免疫组化方法检测抗TGF-β 1抗体和hrTGF-β 1处理后孕鼠子宫内膜组织中MHC I抗原（H2-D1）和MHC II抗原伴侣（H2-DM）的表达。结果显示，与对照组相比，抗TGF-β 1抗体处理的小鼠的植入胚胎数减少。H2-D1和H2-DM在处理后第6天和第7天的表达在mRNA和蛋白水平上均增加。hrTGF-β 1处理组H2-D1和H2-DM蛋白表达降低，植入胚胎数略有增加。免疫组化显示H2-D1和H2-DM主要定位于原代蜕膜区。抗TGF-β 1抗体和外源性hrTGF-β 1处理改变了H2-D1和H2-DM信号的强度，但没有改变它们的定位。这些结果表明，注射抗TGF-β 1抗体影响小鼠胚胎着床数，并调节H2-DM和H2-Q10的表达。
We investigated the mechanism that TGF-beta1 influences the immuno-environment at maternal-fetal interface and affects embryo implantation, using mouse uterine horn injection model. The expression of MHC I antigen (H2-D1) and the chaperone of MHC II antigen (H2-DM) after anti-TGF-beta1 antibody or hrTGF-beta1 treated pregnant mice were examined by real-time PCR, western blotting and immunohistochemistry. The results showed that the number of implanted embryos of anti-TGF-beta1 antibody-treated mice was decreased compared with the control. The expression of H2-D1 and H2-DM on days 6 and 7 treated uteri was increased both at mRNA and protein levels. In hrTGF-beta1 treated group, the expression of H2-D1 and H2-DM protein was decreased, and the number of implanted embryos was slightly increased. Immunohistochemical studies revealed that H2-D1 and H2-DM were mainly localized to the primary decidual zone. The anti-TGF-beta1 antibody and exogenous hrTGF-beta1 treatment altered the intensity of H2-D1 and H2-DM signal but did not change their localization. These observations suggested that injection of anti-TGF-beta1 antibody affected the number of mouse embryo implantation, and regulated the expression of H2-DM and H2-Q10.