Synergy between the phage endolysin LysH5 and nisin to kill Staphylococcus aureus in pasteurized milk
Synergy between the phage endolysin LysH5 and nisin to kill Staphylococcus aureus in pasteurized milk
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DOI：
10.1016/j.ijfoodmicro.2010.04.029
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发表时间：
2010-07-15
影响因子：
5.4
通讯作者：
Rodriguez, Ana
中科院分区：
文献类型：
--
作者：
Garcia, Pilar;Martinez, Beatriz;Rodriguez, Ana
噬菌体编码的内溶素是近年来发现的一种新的生物防治工具。在这项工作中，我们研究了最佳裂解活性的LysH 5，内溶素编码的葡萄球菌噬菌体phi-SauS-IPLA 88的离子要求。Mn（++）和Zn（++）抑制LysH 5活性，Ca（++）、Mg（++）和NaCl增强LysH 5活性。当LysH 5与乳链菌肽（目前用作食品中的生物防腐剂的细菌素）组合时，观察到强烈的协同效应。在棋盘微量滴定试验中测定，乳链菌肽和LysH 5的最低抑制浓度分别降低了64倍和16倍。此外，乳链菌肽增强8倍的裂解活性的LysH 5细胞悬液。在体外观察到的协同作用在被S. aureus Sa9.病原体的清除仅通过两种抗菌剂的联合活性实现。据我们所知，这是第一个利用栅栏技术结合噬菌体编码的内溶素和细菌素乳酸链球菌素的可能性来有效地生产S。牛奶中的金黄色葡萄球菌抑制。(C)2010爱思唯尔有限公司版权所有。
Phage-encoded endolysins are recently considered as new biocontrol tools to inhibit pathogens in food. In this work, we have studied the ionic requirements for optimal lytic activity of LysH5, the endolysin encoded by the staphylococcal bacteriophage phi-SauS-IPLA88. LysH5 activity was inhibited by the presence of Mn(++) and Zn(++) and enhanced by Ca(++), Mg(++) and NaCI. When LysH5 was combined with nisin, a bacteriocin currently used as a biopreservative in food, a strong synergistic effect was observed. The Minimum Inhibitory Concentrations of nisin and LysH5 were reduced 64- and 16-fold, respectively, as determined in checkerboard microtitre tests. In addition, nisin enhanced 8-fold the lytic activity of LysH5 on cell suspensions. The synergy observed in vitro was confirmed in challenge assays in pasteurized milk contaminated with S. aureus Sa9. Clearance of the pathogen was only achieved by the combined activity of both antimicrobials. As far as we know, this is the first study that exploits the possibilities of hurdle technology combining a phage-encoded endolysin and the bacteriocin nisin for efficient S. aureus inhibition in milk. (C) 2010 Elsevier B.V. All rights reserved.