Identification, growth, and pigment content of a spontaneous green mutant of Pyropia kinositae (Bangiales, Rhodophyta).
Identification, growth, and pigment content of a spontaneous green mutant of Pyropia kinositae (Bangiales, Rhodophyta).
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Pyropia kinositae（Bangiales，红藻门）自发绿色突变体的鉴定、生长和色素含量。
DOI：
10.1007/s10811-020-02085-5
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发表时间：
2020
影响因子：
3.3
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Sano;F.;Murata;K. & Niwa;K.
在日本东北部成濑川河口地区的自然种群中发现了野生鹿蹄草的绿色配子体叶片。为了鉴定分离的绿色突变株K-G1和来自同一群体的野生型菌株K-1，我们进行了形态学观察和RUBISCO间隔区和therbcL基因的分子分析。结果表明，这两株菌均为与海洋作物条斑Pyropia yezoensis亲缘关系较近的一种Pyropia kinositae。在相同的实验室培养条件下，比较了绿色突变株K-G1与野生型K-1的生长和色素含量。丝状体和配子体叶片的生长往往是缓慢的绿色突变体比野生型，虽然他们的生长差异并不明显，在一些温度条件下检查。在所研究的温度条件（5-25 °C）中，两个菌株的丝状体和叶片在20 °C下成熟最快。绿色突变体的藻红蛋白含量显著低于野生型。另外，绿色突变体的颜色表型在孢子体丝状体和配子体阶段都是遗传稳定的。由于色素突变体的颜色有利于作为雌雄同株的鹿蹄草属物种的遗传标记进行交叉确认，因此这些结果表明，绿色突变体的P。Kinositae为鹿蹄草属植物的杂交育种和多倍体育种提供了新的育种材料。
A green-colored gametophytic blade of wildPyropiawas found in a natural population in the estuary area of the Naruse River, northeast Japan. To identify the isolated green mutant strain K-G1 and the wild-type strain K-1 derived from the same population, we carried out morphological observations and molecular analysis of the RUBISCO spacer region and therbcL gene. As a result, the two strains were identified asPyropia kinositae, which is a species closely related to the marine cropPyropia yezoensis. Under the same laboratory culture, the growth and pigment contents of the green mutant K-G1 were compared with those of the wild-type K-1. The growth of the conchocelis and gametophytic blade tended to be slower in the green mutant than in the wild type, although differences in their growth were not apparent in some of the temperature conditions examined. Maturation of the conchocelis and blade in the two strains was fastest at 20 °C among the temperature conditions examined (5–25 °C). The phycoerythrin content was significantly lower in the green mutant than in the wild type. Additionally, the color phenotype of the green mutant was genetically stable in both the sporophytic conchocelis and gametophytic phases. Because the color of a pigmentation mutant facilitates cross-confirmation as a genetic marker in monoeciousPyropiaspecies, these results suggest that the green mutant ofP. kinositaewill contribute as new breeding material for cross-breeding and polyploidy breeding inPyropia.